MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
464 323.1118 322.1045
465 323.1122 322.1049
466 323.1124 322.1051
467 323.1469 322.1396
468 323.1470 322.1397
469 323.1472 322.1399
470 323.1473 322.1400
471 323.1473 322.1400
472 323.1474 322.1401
473 323.1475 322.1402
474 323.1475 322.1402
475 323.1477 322.1404
476 323.1478 322.1405
477 323.1480 322.1407
478 323.1480 322.1407
479 323.1482 322.1409
480 323.1484 322.1411
481 323.1487 322.1414
482 323.1490 322.1417
483 323.1491 322.1418
484 323.1495 322.1422
485 323.1496 322.1423
486 323.1678 322.1605
487 323.1843 322.1770
488 323.1846 322.1773
489 323.1847 322.1774
490 323.1851 322.1778
491 323.1851 322.1778
492 323.1851 322.1778
493 323.1852 322.1779
494 323.1852 322.1779
495 323.1855 322.1782
496 323.1855 322.1782
497 323.1857 322.1784
498 323.1860 322.1787
499 323.1861 322.1788
500 323.1862 322.1789
501 323.1862 322.1789
502 323.1863 322.1790
503 323.1863 322.1790
504 323.1864 322.1791
505 323.1865 322.1792
506 323.1866 322.1793
507 323.2043 322.1970
508 323.2060 322.1987
509 324.0300 323.0227
510 324.0314 323.0241
511 325.0607 324.0534
512 325.0890 324.0817
513 325.0902 324.0829
514 325.0956 324.0883
515 325.0971 324.0898
516 325.1262 324.1189
517 325.1263 324.1190
518 325.1266 324.1193
519 325.1269 324.1196
520 325.1269 324.1196
521 325.1272 324.1199
522 325.1273 324.1200
523 325.1273 324.1200
524 325.1273 324.1200
525 325.1275 324.1202
526 325.1276 324.1203
527 325.1278 324.1205
528 325.1281 324.1208
529 325.1286 324.1213
530 325.1632 324.1559
531 325.1636 324.1563
532 325.1638 324.1565
533 325.1638 324.1565
534 325.1638 324.1565
535 325.1643 324.1570
536 325.1644 324.1571
537 325.1652 324.1579
538 325.1654 324.1581
539 325.1654 324.1581
540 325.1658 324.1585
541 325.1822 324.1749
542 325.1828 324.1755
543 325.1831 324.1758
544 325.1836 324.1763
545 325.2021 324.1948
546 325.2027 324.1954
547 325.2027 324.1954
548 325.2028 324.1955
549 325.2029 324.1956
550 325.2029 324.1956
551 325.2030 324.1957
552 325.2031 324.1958
553 325.2033 324.1960
554 325.2204 324.2131
555 325.2215 324.2142
556 326.0458 325.0385
557 326.0459 325.0386
558 326.0459 325.0386
559 326.0463 325.0390
560 326.0463 325.0390
561 326.0471 325.0398
562 326.1968 325.1895
563 326.1972 325.1899
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