MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 7858 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 967 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_ECOLI | 915 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TKT1_ECOLI | 376 | Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 289 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | AMPA_ECOLI |
289 | 55358 | 11 (9) | 11 (9) | 0.68 | |
| Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 | |||||||
11 peptide matches (11 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 2194 |
301.6864 | 601.3582 | 601.3548 | 5.81 | 0 | 3 | 1.1 | 2Score > 21 indicates identity Score > 16 indicates homology |
U | K.NVITR.V | |
| 2412 |
437.2656 | 872.5166 | 872.5154 | 1.49 | 0 | 42 | 0.00014 | 1Score > 16 indicates identity |
U | R.ILLIGCGK.E | |
| 2438 |
456.2285 | 910.4424 | 910.4396 | 3.14 | 0 | 48 | 3.6e-005 | 1Score > 16 indicates identity |
U | R.QLADSYSK.N | |
| 2620 |
557.3149 | 1112.6152 | 1112.6077 | 6.77 | 0 | 12 | 0.22 | 1Score > 17 indicates identity |
U | R.LSPIAEQLDK.I | |
| 2716 |
604.3425 | 1206.6704 | 1206.6608 | 7.99 | 0 | 64 | 7.1e-007 | 1Score > 15 indicates identity |
U | K.ISDGYISALLR.R | |
| 2756 |
622.3168 | 1242.6190 | 1242.6132 | 4.70 | 0 | 34 | 0.0015 | 1Score > 18 indicates identity |
U | K.ETLYSFDQLK.T | |
| 2770 |
421.5926 | 1261.7560 | 1261.7506 | 4.24 | 0 | 46 | 2.2e-005 | 1Score > 13 indicates identity |
U | R.AIQHGLAIAAGIK.A | |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube1_A1.rawScore > 16 indicates identity |
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| 2971 |
740.4063 | 1478.7980 | 1478.7803 | 12.0 | 0 | 52 | 1.7e-005 | 1Score > 16 indicates identity |
U | R.LVLCDVLTYVER.F | |
| No other peptide matches in query | |||||||||||
| 3039 |
509.2857 | 1524.8353 | 1524.8260 | 6.09 | 0 | 26 | 0.0046 | 1Score > 16 indicates identity |
U | K.GNASEDARPIVLVGK.G | |
| 3585 |
768.4334 | 2302.2784 | 2302.2420 | 15.8 | 1 | 23 | 0.0052 | 1Score > 13 indicates identity |
U | R.LSPIAEQLDKISDGYISALLR.R + Deamidated (NQ) | |
| 3956 |
933.1246 | 2796.3520 | 2796.3236 | 10.1 | 0 | 80 | 4.6e-008 | 1Score > 19 indicates identity |
U | R.AYRPGDVLTTMSGQTVEVLNTDAEGR.L + Deamidated (NQ); Oxidation (M) | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 110 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLB_ECOLI | 81 | Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABZ_ECOLI | 73 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 69 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
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