MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (45 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 DNAK_ECOLI 7858 69130 293 (254) 65 (60) 206.58 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2
2 RHAA_ECOLI 967 47455 36 (30) 23 (20) 4.36 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2
3 ODP2_ECOLI 915 66112 44 (32) 34 (27) 3.28 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3
4 TKT1_ECOLI 376 72451 21 (14) 20 (14) 0.86 Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5
5 AMPA_ECOLI 289 55358 11 (9) 11 (9) 0.68 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1
6 TRYP_PIG 261 25078 12 (8) 4 (4) 0.87 Trypsin OS=Sus scrofa PE=1 SV=1
7 CATNterm_ECOLX 110 27452 6 (4) 4 (3) 0.58 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1
8 XYLB_ECOLI 81 52927 3 (2) 2 (2) 0.13 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1
9 FABZ_ECOLI 73 17136 4 (3) 4 (3) 0.72 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1
10 CH60_ECOLI 69 57464 5 (2) 5 (2) 0.12 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
11 PEPB_ECOLI 69 46436 5 (4) 5 (4) 0.32 Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2
12 POXB_ECOLI 65 62542 3 (3) 3 (3) 0.17 Pyruvate dehydrogenase [ubiquinone] OS=Escherichia coli (strain K12) GN=poxB PE=1 SV=1
13 SDHA_ECOLI 61 65008 2 (2) 2 (2) 0.10 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1
14 IPYR_ECOLI 51 19805 2 (2) 2 (2) 0.37 Inorganic pyrophosphatase OS=Escherichia coli (strain K12) GN=ppa PE=1 SV=2
15 RPOB_ECOLI 50 150937 4 (1) 4 (1) 0.02 DNA-directed RNA polymerase subunit beta OS=Escherichia coli (strain K12) GN=rpoB PE=1 SV=1
16 K2C1_HUMAN 49 66170 1 (1) 1 (1) 0.05 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
17 ATPB_ECOLI 49 50351 1 (1) 1 (1) 0.07 ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2
18 ADHP_ECOLI 47 35870 3 (2) 2 (1) 0.19 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1
19 LPP_ECOLI 43 8375 2 (1) 2 (1) 0.42 Major outer membrane lipoprotein Lpp OS=Escherichia coli (strain K12) GN=lpp PE=1 SV=1
20 Q835L3_ENTFA 42 86643 3 (1) 2 (1) 0.04 Acetyl-CoA acetyltransferase/hydroxymethylglutaryl-CoA reductase, degradative OS=Enterococcus faecalis (strain ATCC 700802 / V583) GN=EF_1364 PE=3 SV=1
21 HFQ_ECOLI 40 11160 2 (1) 2 (1) 0.31 RNA-binding protein Hfq OS=Escherichia coli (strain K12) GN=hfq PE=1 SV=2
22 Q6E7K0_9CYAN 39 366003 6 (1) 5 (1) 0.01 3-hydroxyacyl-CoA dehydrogenase OS=Lyngbya majuscula OX=158786 PE=1 SV=1
23 YQJC_ECOLI 35 0 2 (2) 1 (1) 0.25 description
24 LONH_ECOLI 35 0 2 (2) 1 (1) 0.05 description
25 F4JF56_ARATH 31 48926 1 (1) 1 (1) 0.07 Sialyltransferase-like protein OS=Arabidopsis thaliana GN=At3g48820 PE=4 SV=1
26 YCDX_ECOLI 29 27044 2 (1) 2 (1) 0.12 Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1
27 RL5_ECOLI 29 20346 1 (1) 1 (1) 0.17 50S ribosomal protein L5 OS=Escherichia coli (strain K12) GN=rplE PE=1 SV=2
28 FADB_ECOLI 27 0 2 (1) 1 (1) 0.04 description
29 GLRR_ECOLI 27 49232 1 (1) 1 (1) 0.07 Transcriptional regulatory protein GlrR OS=Escherichia coli (strain K12) GN=glrR PE=1 SV=1
30 YPJA_ECOLI 26 157576 2 (1) 2 (1) 0.02 Uncharacterized outer membrane protein YpjA OS=Escherichia coli (strain K12) GN=ypjA PE=4 SV=3
31 ENTC_ECOLI 26 43133 2 (1) 2 (1) 0.08 Isochorismate synthase EntC OS=Escherichia coli (strain K12) GN=entC PE=1 SV=1
32 SODF_ECOLI 25 21310 1 (1) 1 (1) 0.16 Superoxide dismutase [Fe] OS=Escherichia coli (strain K12) GN=sodB PE=1 SV=2
33 YGDH_ECOLI 23 0 2 (1) 1 (1) 0.07 description
34 PAAC_ECOLI 21 27974 1 (1) 1 (1) 0.12 1,2-phenylacetyl-CoA epoxidase, subunit C OS=Escherichia coli (strain K12) GN=paaC PE=1 SV=1
35 GUAA_ECOLI 21 59041 2 (1) 2 (1) 0.06 GMP synthase [glutamine-hydrolyzing] OS=Escherichia coli (strain K12) GN=guaA PE=1 SV=1
36 NAGA_ECOLI 20 41379 3 (1) 1 (1) 0.08 N-acetylglucosamine-6-phosphate deacetylase OS=Escherichia coli (strain K12) GN=nagA PE=1 SV=1
37 MCP2_ECOLI 20 59907 1 (1) 1 (1) 0.05 Methyl-accepting chemotaxis protein II OS=Escherichia coli (strain K12) GN=tar PE=1 SV=2
38 PRUB680 20 0 1 (1) 1 (1) 0.07 description
39 PERM_ECOLI 19 39283 1 (1) 1 (1) 0.08 Putative permease PerM OS=Escherichia coli (strain K12) GN=perM PE=3 SV=1
40 DHE3_BOVIN 18 61815 5 (1) 2 (1) 0.05 Glutamate dehydrogenase 1, mitochondrial OS=Bos taurus GN=GLUD1 PE=1 SV=2
41 LIGB_ECOLI 18 63425 1 (1) 1 (1) 0.05 DNA ligase B OS=Escherichia coli (strain K12) GN=ligB PE=1 SV=2
42 YBFC_ECOLI 18 22175 1 (1) 1 (1) 0.15 Uncharacterized protein YbfC OS=Escherichia coli (strain K12) GN=ybfC PE=4 SV=1
43 HUMS_ARATH 17 0 2 (1) 1 (1) 0.05 description
44 NIKA_ECOLI 15 58739 3 (1) 3 (1) 0.06 Nickel-binding periplasmic protein OS=Escherichia coli (strain K12) GN=nikA PE=1 SV=1
45 FABF_ECOLI 14 43247 1 (1) 1 (1) 0.08 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Escherichia coli (strain K12) GN=fabF PE=1 SV=2

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