MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 45)

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+1

Accession Score Description
1 DNAK_ECOLI 7858 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2

+2

Accession Score Description
1 RHAA_ECOLI 967 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2

-3

Accession Score Description
1 ODP2_ECOLI 915 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3
Score Mass Matches Sequences emPAI
3.1 ODP2_ECOLI 915 66112 44 (32) 34 (27) 3.28
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3

-44 peptide matches (40 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2185   573.3634 572.3561 572.3533 4.88 0 12 0.25 +1Score > 19 indicates identity U M.AIEIK.V
2196   301.7163 601.4180 601.4163 2.95 1 5 0.32 +1Score > 13 indicates identity U R.LTLKK.Y
2234   324.1799 646.3452 646.3439 2.15 0 37 0.00069 +1Score > 18 indicates identity U K.EFVPR.L
2348   394.2375 786.4604 786.4599 0.66 0 17 0.1 +1Score > 19 indicates identity U K.GIIELSR.E
2375   409.2303 816.4460 816.4453 0.86 0 40 0.00052 +1Score > 20 indicates identity U K.ISGANLSR.N
2385   419.2237 836.4328 836.4313 1.81 0 35 0.0008 +1Score > 17 indicates identity U R.ELMTISK.K + Oxidation (M)
2400   429.7573 857.5000 857.4971 3.47 1 15 0.15 +1Score > 20 indicates identity U K.VNVGDKVK.T
2410   437.2272 872.4398 872.4352 5.33 0 47 7.3e-005 +1Score > 18 indicates identity U R.VIDGADGAR.F
2415   439.2438 876.4730 876.4705 2.90 0 49 3.1e-005 +1Score > 16 indicates identity U R.EFGVNLAK.V
2467   476.2439 950.4732 950.4709 2.47 0 56 7.9e-006 +1Score > 18 indicates identity U R.EDVQAYVK.E
2482   484.2639 966.5132 966.5134 -0.15 0 46 6.7e-005 +1Score > 16 indicates identity U K.AEAPAAAPAAK.A
2550   512.7763 1023.5380 1023.5349 3.11 0 55 6.8e-006 +1Score > 16 indicates identity U K.QEAAAPAPAAK.A
2580 +1 532.3160 1062.6174 1062.6147 2.54 0 46 2.4e-005 +1Score > 13 indicates identity U K.ITPVVFIMK.A + Oxidation (M)
2661   586.8101 1171.6056 1171.6019 3.19 0 51 2.8e-005 +1Score > 18 indicates identity U K.AVAAALEQMPR.F + Oxidation (M)
2662   391.5435 1171.6087 1171.6019 5.77 0 4 1.4 +3Score > 18 indicates identity U K.AVAAALEQMPR.F + Oxidation (M)
2663   587.2916 1172.5686 1172.5859 -14.7 0 0 2.2 +2Score > 16 indicates identity U K.AVAAALEQMPR.F + Deamidated (NQ); Oxidation (M)
2676   590.8251 1179.6356 1179.6247 9.26 0 2 1.2 +1Score > 16 indicates identity U K.EAAPAAAPAAAAAK.D
2752   620.2869 1238.5592 1238.5527 5.26 0 82 1.4e-008 +1Score > 16 indicates identity U R.FNSSLSEDGQR.L
2786 +1 639.3267 1276.6388 1276.6299 7.00 0 75 1.6e-007 +1Score > 19 indicates identity U K.FGEIEEVELGR.I
2822   436.9145 1307.7217 1307.7197 1.52 1 48 2.9e-005 +1Score > 15 indicates identity U K.KEAAPAAAPAAAAAK.D
2835   441.5716 1321.6930 1321.6878 3.94 1 36 0.00086 +1Score > 18 indicates identity U K.TDITELEAFRK.Q
2846   445.2544 1332.7414 1332.7401 0.93 1 11 0.19 +1Score > 17 indicates identity U R.ILREDVQAYVK.E
2918   473.9252 1418.7538 1418.7517 1.43 0 4 2 +2Score > 19 indicates identity U K.QEAAPAAAPAPAAGVK.E
2919   710.3861 1418.7576 1418.7517 4.16 0 57 9.1e-006 +1Score > 19 indicates identity U K.QEAAPAAAPAPAAGVK.E
2946 +1 482.2614 1443.7624 1443.7544 5.51 0 60 3.7e-006 +1Score > 18 indicates identity U R.LMLPISLSFDHR.V + Oxidation (M)
3031   760.4340 1518.8534 1518.8406 8.47 0 78 2.1e-008 +1Score > 14 indicates identity U R.FITIINNTLSDIR.R
3180   838.4836 1674.9526 1674.9417 6.55 1 14 0.039 +1Score > 13 indicates identity U R.FITIINNTLSDIRR.L
3184 +1 559.6578 1675.9516 1675.9257 15.4 1 34 0.00064 +1Score > 14 indicates identity U R.FITIINNTLSDIRR.L + Deamidated (NQ)
3294   614.0054 1838.9944 1838.9877 3.63 0 39 0.00032 +1Score > 16 indicates identity U K.VPDIGADEVEITEILVK.V
3342   645.3229 1932.9469 1932.9217 13.0 0 56 1.3e-005 +1Score > 19 indicates identity U K.SEFAENDAYVHATPLIR.R + Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube1_A1.raw

Score > 18 indicates identity
3397   687.3712 2059.0918 2059.0659 12.5 0 32 0.0021 -1Score > 18 indicates identity U K.TGSLIMIFEVEGAAPAAAPAK.Q + Oxidation (M)
No other peptide matches in query
3475   1086.0696 2170.1246 2170.0893 16.3 0 29 0.0048 +1Score > 18 indicates identity U K.DVNVPDIGSDEVEVTEILVK.V + Deamidated (NQ)
3476   724.3845 2170.1317 2170.0893 19.5 0 27 0.0063 +1Score > 17 indicates identity U K.DVNVPDIGSDEVEVTEILVK.V + Deamidated (NQ)
3483   725.6929 2174.0569 2174.0301 12.3 0 26 0.013 +1Score > 19 indicates identity U K.EVNVPDIGGDEVEVTEVMVK.V + Deamidated (NQ); Oxidation (M)
3484   1088.0371 2174.0596 2174.0301 13.6 0 39 0.00055 +1Score > 19 indicates identity U K.EVNVPDIGGDEVEVTEVMVK.V + Deamidated (NQ); Oxidation (M)
3596   580.5436 2318.1453 2318.1178 11.8 1 28 0.0092 +1Score > 20 indicates identity U K.AEGKSEFAENDAYVHATPLIR.R + Deamidated (NQ)
3981   946.4675 2836.3807 2836.3436 13.1 1 67 6e-007 +1Score > 20 indicates identity
Score > 17 indicates homology		 U K.TQTGALIMIFDSADGAADAAPAQAEEKK.E + Deamidated (NQ); Oxidation (M)
4003   959.5102 2875.5088 2875.4637 15.7 1 52 1.9e-005 +1Score > 17 indicates identity U K.VNVGDKVSTGSLIMVFEVAGEAGAAAPAAK.Q + Deamidated (NQ); Oxidation (M)
4079   1022.8773 3065.6101 3065.5743 11.7 1 36 0.00066 +1Score > 16 indicates identity U K.TGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAK.A + Deamidated (NQ); Oxidation (M)
4150   1098.8690 3293.5852 3293.5609 7.37 1 11 0.43 +1Score > 19 indicates identity U K.VSVGDKTQTGALIMIFDSADGAADAAPAQAEEK.K + Deamidated (NQ); Oxidation (M)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
UPI0002BC832D 37 0 3.1
description

+4

Accession Score Description
1 TKT1_ECOLI 376 Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5

+5

Accession Score Description
1 AMPA_ECOLI 289 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1

+6

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 CATNterm_ECOLX 110 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1

+8

Accession Score Description
1 XYLB_ECOLI 81 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1

+9

Accession Score Description
1 FABZ_ECOLI 73 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1

+10

Accession Score Description
1 CH60_ECOLI 69 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
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