MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 45)

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+1

Accession Score Description
1 DNAK_ECOLI 7858 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2

+2

Accession Score Description
1 RHAA_ECOLI 967 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2

-3

Accession Score Description
1 ODP2_ECOLI 915 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3
Score Mass Matches Sequences emPAI
3.1 ODP2_ECOLI 915 66112 44 (32) 34 (27) 3.28
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3

+44 peptide matches (40 non-duplicate, 4 duplicate)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
UPI0002BC832D 37 0 3.1
description

+4

Accession Score Description
1 TKT1_ECOLI 376 Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5

+5

Accession Score Description
1 AMPA_ECOLI 289 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1

+6

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 CATNterm_ECOLX 110 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1

+8

Accession Score Description
1 XYLB_ECOLI 81 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1

+9

Accession Score Description
1 FABZ_ECOLI 73 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1

+10

Accession Score Description
1 CH60_ECOLI 69 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
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