MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 45)

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+21

Accession Score Description
1 HFQ_ECOLI 40 RNA-binding protein Hfq OS=Escherichia coli (strain K12) GN=hfq PE=1 SV=2

-22

Accession Score Description
1 Q6E7K0_9CYAN 39 3-hydroxyacyl-CoA dehydrogenase OS=Lyngbya majuscula OX=158786 PE=1 SV=1
Score Mass Matches Sequences emPAI
22.1 Q6E7K0_9CYAN 39 366003 6 (1) 5 (1) 0.01
3-hydroxyacyl-CoA dehydrogenase OS=Lyngbya majuscula OX=158786 PE=1 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2147 +1 545.3326 544.3253 544.3221 6.01 0 39 0.0015 +1Score > 24 indicates identity U K.GLQVK.L + Deamidated (NQ)
2244   660.3946 659.3873 659.3854 2.95 0 11 0.23 +1Score > 17 indicates identity U K.LLADTK.E
2533   505.2611 1008.5076 1008.5063 1.38 0 1 3.9 +4Score > 19 indicates identity K.QFCAIGSVK.T
2596   1081.5024 1080.4951 1080.5087 -12.6 0 5 0.89 +2Score > 17 indicates identity U R.TTEFANEIR.E + Deamidated (NQ)
2916   709.3760 1416.7374 1416.7249 8.89 0 11 0.36 +3Score > 19 indicates identity U K.LWSGSNQALAEIK.L + Deamidated (NQ)

+23

Accession Score Description
1 YQJC_ECOLI 35 description

+24

Accession Score Description
1 LONH_ECOLI 35 description

+25

Accession Score Description
1 F4JF56_ARATH 31 Sialyltransferase-like protein OS=Arabidopsis thaliana GN=At3g48820 PE=4 SV=1

+26

Accession Score Description
1 YCDX_ECOLI 29 Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1

+27

Accession Score Description
1 RL5_ECOLI 29 50S ribosomal protein L5 OS=Escherichia coli (strain K12) GN=rplE PE=1 SV=2

+28

Accession Score Description
1 FADB_ECOLI 27 description

+29

Accession Score Description
1 GLRR_ECOLI 27 Transcriptional regulatory protein GlrR OS=Escherichia coli (strain K12) GN=glrR PE=1 SV=1

+30

Accession Score Description
1 YPJA_ECOLI 26 Uncharacterized outer membrane protein YpjA OS=Escherichia coli (strain K12) GN=ypjA PE=4 SV=3
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