MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
187 309.1687 308.1614
188 309.1687 308.1614
189 309.1689 308.1616
190 309.1690 308.1617
191 309.1694 308.1621
192 309.1697 308.1624
193 309.1698 308.1625
194 309.1702 308.1629
195 309.1706 308.1633
196 309.1708 308.1635
197 309.1892 308.1819
198 309.1893 308.1820
199 309.1894 308.1821
200 309.2063 308.1990
201 309.2067 308.1994
202 309.2069 308.1996
203 309.2074 308.2001
204 309.2074 308.2001
205 309.2075 308.2002
206 309.2076 308.2003
207 309.2081 308.2008
208 310.0146 309.0073
209 310.0150 309.0077
210 311.0742 310.0669
211 311.0811 310.0738
212 311.0825 310.0752
213 311.0906 310.0833
214 311.1105 310.1032
215 311.1107 310.1034
216 311.1107 310.1034
217 311.1111 310.1038
218 311.1112 310.1039
219 311.1112 310.1039
220 311.1113 310.1040
221 311.1117 310.1044
222 311.1118 310.1045
223 311.1468 310.1395
224 311.1473 310.1400
225 311.1474 310.1401
226 311.1475 310.1402
227 311.1476 310.1403
228 311.1476 310.1403
229 311.1476 310.1403
230 311.1478 310.1405
231 311.1479 310.1406
232 311.1480 310.1407
233 311.1483 310.1410
234 311.1487 310.1414
235 311.1488 310.1415
236 311.1490 310.1417
237 311.1493 310.1420
238 311.1495 310.1422
239 311.1671 310.1598
240 311.1672 310.1599
241 311.1679 310.1606
242 311.2053 310.1980
243 311.2057 310.1984
244 311.2058 310.1985
245 311.2059 310.1986
246 311.2060 310.1987
247 311.2234 310.2161
248 311.2239 310.2166
249 312.0301 311.0228
250 312.0303 311.0230
251 312.0305 311.0232
252 312.0307 311.0234
253 312.0307 311.0234
254 312.0310 311.0237
255 313.0597 312.0524
256 313.0898 312.0825
257 313.0968 312.0895
258 313.0984 312.0911
259 313.1266 312.1193
260 313.1266 312.1193
261 313.1267 312.1194
262 313.1269 312.1196
263 313.1275 312.1202
264 313.1289 312.1216
265 313.1289 312.1216
266 313.1292 312.1219
267 313.1628 312.1555
268 313.1635 312.1562
269 313.1647 312.1574
270 313.1656 312.1583
271 313.1847 312.1774
272 313.1858 312.1785
273 313.2213 312.2140
274 313.2247 312.2174
275 313.2387 312.2314
276 314.0099 313.0026
277 314.0100 313.0027
278 314.0454 313.0381
279 314.0457 313.0384
280 314.0459 313.0386
281 314.0459 313.0386
282 314.0459 313.0386
283 314.0461 313.0388
284 314.0465 313.0392
285 314.1967 313.1894
286 314.1971 313.1898
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