MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
87 305.1376 304.1303
88 305.1378 304.1305
89 305.1380 304.1307
90 305.1382 304.1309
91 305.1390 304.1317
92 305.1737 304.1664
93 305.1738 304.1665
94 305.1738 304.1665
95 305.1740 304.1667
96 305.1740 304.1667
97 305.1743 304.1670
98 305.1744 304.1671
99 305.1745 304.1672
100 305.1746 304.1673
101 305.1750 304.1677
102 305.1750 304.1677
103 305.1750 304.1677
104 305.1752 304.1679
105 305.1753 304.1680
106 305.1755 304.1682
107 305.2107 304.2034
108 305.9589 304.9516
109 306.1547 305.1474
110 306.1556 305.1483
111 306.1558 305.1485
112 306.1560 305.1487
113 306.1924 305.1851
114 306.1925 305.1852
115 306.1931 305.1858
116 306.8933 305.8860
117 306.9636 305.9563
118 306.9650 305.9577
119 307.1154 306.1081
120 307.1158 306.1085
121 307.1160 306.1087
122 307.1161 306.1088
123 307.1161 306.1088
124 307.1161 306.1088
125 307.1163 306.1090
126 307.1164 306.1091
127 307.1164 306.1091
128 307.1166 306.1093
129 307.1171 306.1098
130 307.1172 306.1099
131 307.1526 306.1453
132 307.1526 306.1453
133 307.1527 306.1454
134 307.1529 306.1456
135 307.1530 306.1457
136 307.1530 306.1457
137 307.1530 306.1457
138 307.1531 306.1458
139 307.1531 306.1458
140 307.1533 306.1460
141 307.1533 306.1460
142 307.1539 306.1466
143 307.1539 306.1466
144 307.1539 306.1466
145 307.1541 306.1468
146 307.1547 306.1474
147 307.1886 306.1813
148 307.1901 306.1828
149 307.1902 306.1829
150 307.1906 306.1833
151 307.1906 306.1833
152 307.1906 306.1833
153 307.1909 306.1836
154 307.1909 306.1836
155 307.1909 306.1836
156 307.1910 306.1837
157 307.1911 306.1838
158 307.1912 306.1839
159 307.1913 306.1840
160 307.1916 306.1843
161 307.1920 306.1847
162 307.2101 306.2028
163 308.0359 307.0286
164 309.0947 308.0874
165 309.0950 308.0877
166 309.0954 308.0881
167 309.0956 308.0883
168 309.1312 308.1239
169 309.1315 308.1242
170 309.1317 308.1244
171 309.1318 308.1245
172 309.1318 308.1245
173 309.1319 308.1246
174 309.1320 308.1247
175 309.1320 308.1247
176 309.1320 308.1247
177 309.1322 308.1249
178 309.1324 308.1251
179 309.1325 308.1252
180 309.1327 308.1254
181 309.1328 308.1255
182 309.1678 308.1605
183 309.1683 308.1610
184 309.1684 308.1611
185 309.1685 308.1612
186 309.1686 308.1613
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