| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 696.6773 | 2087.0101 | 2086.9953 | 7.08 | 1 | 1 | 2.9 | 1Score > 18 indicates identity |
MLDGGDNPLRVQIGSNNIR + 3 Deamidated (NQ); Oxidation (M) | |
| 716.8740 | 2863.4669 | 2863.4637 | 1.12 | 1 | 1 | 3.1 | 1Score > 19 indicates identity |
STLMKIIAGITPADSGTLEIEGNNYVR + Deamidated (NQ) | |
| 1149.5731 | 3445.6975 | 3445.7221 | -7.15 | 0 | 1 | 2.6 | 1Score > 18 indicates identity |
AGLTMSDLTLIDMHEAFAAQTLANIQLLGSER + Oxidation (M) | |
| 580.3214 | 2317.2565 | 2317.2426 | 6.02 | 1 | 1 | 1.8 | 1Score > 16 indicates identity |
NVTFTTINMPLVKTPMIAPTK + Deamidated (NQ) | |
| 485.9577 | 1454.8513 | 1454.8279 | 16.1 | 1 | 1 | 0.76 | 1Score > 13 indicates identity |
LMRLIQANPALSK + Deamidated (NQ) | |
| 502.9029 | 1505.6869 | 1505.7110 | -16.0 | 1 | 1 | 2.8 | 1Score > 18 indicates identity |
LIANRNDDAAFER + 2 Deamidated (NQ) | |
| 590.2770 | 1178.5394 | 1178.5608 | -18.1 | 0 | 1 | 2.1 | 1Score > 17 indicates identity |
GAWLEFDVDK | |
| 947.9961 | 1893.9776 | 1893.9544 | 12.3 | 1 | 1 | 3.2 | 1Score > 19 indicates identity |
VHRGDLVVEGNIESNQK + Deamidated (NQ) | |
| 638.0541 | 2548.1873 | 2548.1971 | -3.86 | 1 | 1 | 3.4 | 1Score > 19 indicates identity |
SSSVETMPDLPLKVMMNVGNPDR + 2 Oxidation (M) | |
| 525.7671 | 1049.5196 | 1049.5294 | -9.32 | 0 | 1 | 0.84 | 1Score > 20 indicates identityScore > 13 indicates homology |
QWVFSEVR | |
| 1088.7531 | 4350.9833 | 4350.9208 | 14.4 | 0 | 1 | 1.9 | 1Score > 16 indicates identity |
MVGQTAQWWLEYHDNQLTSLINNALQNAPDMQVAEQR + 7 Deamidated (NQ); 2 Oxidation (M) | |
| 797.9064 | 1593.7982 | 1593.8111 | -8.03 | 1 | 1 | 3.1 | 1Score > 19 indicates identity |
ALSREYAQLSDVSR | |
| 455.2626 | 908.5106 | 908.4967 | 15.3 | 0 | 1 | 1 | 1Score > 14 indicates identity |
IAVSYQTK | |
| 336.8547 | 1007.5423 | 1007.5287 | 13.5 | 1 | 1 | 2.4 | 1Score > 17 indicates identity |
EKAEGLYAK | |
| 609.9697 | 1826.8873 | 1826.8758 | 6.27 | 1 | 1 | 3.6 | 1Score > 19 indicates identity |
AIDNQIRNISSSHQDK + 2 Deamidated (NQ) | |
| 720.8727 | 2879.4617 | 2879.4916 | -10.4 | 0 | 1 | 3.4 | 1Score > 19 indicates identity |
NLVLDIDYNDAVVVIHTSPGAAQLIAR + 3 Deamidated (NQ) | |
| 313.1507 | 624.2868 | 624.2867 | 0.19 | 0 | 1 | 2 | 1Score > 16 indicates identity |
DAYTR | |
| 508.9044 | 1523.6914 | 1523.6674 | 15.7 | 0 | 1 | 1.8 | 1Score > 16 indicates identity |
YSQQQLMETSHR + Deamidated (NQ); Oxidation (M) | |
| 467.2340 | 1864.9069 | 1864.8724 | 18.5 | 1 | 1 | 3.9 | 1Score > 19 indicates identity |
MSQETPASTTEAQIKNK + 2 Deamidated (NQ) | |
| 329.1722 | 656.3298 | 656.3242 | 8.65 | 0 | 1 | 0.99 | 1Score > 13 indicates identity |
QDAAPR | |
| 415.2169 | 2071.0481 | 2071.0433 | 2.33 | 0 | 1 | 3.9 | 1Score > 19 indicates identity |
LAQLQVAIQNVTQEQTQR + 4 Deamidated (NQ) | |
| 476.5673 | 1426.6801 | 1426.6776 | 1.77 | 1 | 1 | 2.9 | 1Score > 18 indicates identity |
HCVWEEVARNK | |
| 314.7124 | 627.4102 | 627.4180 | -12.4 | 1 | 1 | 1.2 | 1Score > 14 indicates identity |
ARILR | |
| 886.8189 | 4429.0581 | 4429.0988 | -9.18 | 1 | 1 | 2.8 | 1Score > 18 indicates identity |
NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDR + 3 Deamidated (NQ); 2 Oxidation (M) | |
| 586.9587 | 1757.8543 | 1757.8770 | -13.0 | 1 | 1 | 3.4 | 1Score > 19 indicates identity |
LIFMGVEHTQPEKGR + Deamidated (NQ); Oxidation (M) | |
| 1004.8757 | 3011.6053 | 3011.5491 | 18.7 | 0 | 1 | 1.6 | 1Score > 16 indicates identity |
QVNIVINNLLYNPQAVAQLLAWLNER + 6 Deamidated (NQ) | |
| 599.3047 | 1196.5948 | 1196.6077 | -10.8 | 0 | 1 | 2.8 | 1Score > 18 indicates identity |
VQFSSAIGPYK + Deamidated (NQ) | |
| 605.8079 | 1209.6012 | 1209.6063 | -4.20 | 1 | 1 | 2.6 | 1Score > 18 indicates identity |
KLAVMNIEYN + Oxidation (M) | |
| 775.7261 | 2324.1565 | 2324.1120 | 19.1 | 1 | 1 | 3.5 | 1Score > 19 indicates identity |
SHVWGLFSHPDREMQVINR + Deamidated (NQ); Oxidation (M) | |
| 850.7361 | 2549.1865 | 2549.1812 | 2.09 | 1 | 1 | 3.5 | 1Score > 19 indicates identity |
SSSVETMPDLPLKVMMNVGNPDR + Deamidated (NQ); 2 Oxidation (M) | |
| 694.8730 | 4163.1943 | 4163.1248 | 16.7 | 1 | 1 | 1.3 | 1Score > 15 indicates identity |
IRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALER + 2 Deamidated (NQ) | |
| 798.4057 | 1594.7968 | 1594.7773 | 12.3 | 1 | 1 | 3.1 | 1Score > 18 indicates identity |
EMNQQITNYIRGK + Deamidated (NQ) | |
| 604.9687 | 1811.8843 | 1811.8723 | 6.59 | 1 | 1 | 3.4 | 1Score > 19 indicates identity |
AAMDRNYLASVLAGSGLS + Deamidated (NQ); Oxidation (M) | |
| 409.7149 | 817.4152 | 817.4116 | 4.44 | 0 | 1 | 0.89 | 1Score > 20 indicates identityScore > 13 indicates homology |
DAMVGAVR | |
| 669.6867 | 2006.0383 | 2006.0698 | -15.7 | 1 | 1 | 3.4 | 1Score > 19 indicates identity |
GVNVVALYGGQRYDVQLR | |
| 606.3170 | 1210.6194 | 1210.6016 | 14.7 | 0 | 1 | 2.9 | 1Score > 18 indicates identity |
VMQAGAAGYLSK + Oxidation (M) | |
| 464.2664 | 926.5182 | 926.5073 | 11.9 | 0 | 1 | 1.2 | 1Score > 14 indicates identity |
LAEQPIEK | |
| 1018.5356 | 3052.5850 | 3052.5678 | 5.62 | 0 | 1 | 2 | 1Score > 16 indicates identity |
ALAMEPEVLLFDEPTSALDPELVGEVLR | |
| 484.2561 | 966.4976 | 966.4957 | 2.05 | 1 | 1 | 2 | 1Score > 16 indicates identity |
LFSGMQRK + Deamidated (NQ) | |
| 670.7020 | 2009.0842 | 2009.0542 | 14.9 | 1 | 1 | 2.2 | 1Score > 17 indicates identity |
VHTSKNTLEIVQEGVEAR | |
| 492.9330 | 2951.5543 | 2951.5651 | -3.65 | 1 | 1 | 2.3 | 1Score > 17 indicates identity |
FSIALLNQAVERVHTAIVVQDPTMQR + Oxidation (M) | |
| 797.3944 | 1592.7742 | 1592.7980 | -14.9 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
LVERYLAEQAMDR | |
| 898.7599 | 2693.2579 | 2693.2539 | 1.47 | 0 | 1 | 2.7 | 1Score > 18 indicates identity |
IAWLMSVENCSPYSIMAVTFTNK + 2 Oxidation (M) | |
| 390.5388 | 1168.5946 | 1168.5876 | 5.93 | 0 | 1 | 1.9 | 1Score > 16 indicates identity |
QNFELLYSR | |
| 646.6993 | 1937.0761 | 1937.0442 | 16.4 | 1 | 1 | 1.7 | 1Score > 15 indicates identity |
VQLAQEGLGIEAQARQAR | |
| 522.5154 | 2086.0325 | 2086.0596 | -13.0 | 0 | 1 | 3.7 | 1Score > 19 indicates identity |
TAAEYQPLIIHYNNGGAVR | |
| 856.4301 | 2566.2685 | 2566.2461 | 8.73 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
IAIECHSCIQWLTPALENFHK | |
| 621.0681 | 2480.2433 | 2480.2077 | 14.3 | 1 | 1 | 3.5 | 1Score > 19 indicates identity |
TTQISARMNNEGAILANEFSASR | |
| 383.8453 | 1148.5141 | 1148.4954 | 16.2 | 0 | 1 | 1.5 | 1Score > 15 indicates identity |
VMMNVGNPDR + Deamidated (NQ); Oxidation (M) | |
| 509.6044 | 1525.7914 | 1525.8180 | -17.5 | 1 | 1 | 3.4 | 1Score > 18 indicates identity |
FVIADYFINKAPK + Deamidated (NQ) | |
| 506.5102 | 2022.0117 | 2022.0204 | -4.32 | 1 | 1 | 4.3 | 1Score > 20 indicates identity |
QDVSGFSPVLTVNMRAASK + Oxidation (M) | |
| 415.2151 | 2071.0391 | 2071.0433 | -2.01 | 0 | 1 | 4.3 | 1Score > 20 indicates identity |
LAQLQVAIQNVTQEQTQR + 4 Deamidated (NQ) | |
| 716.8727 | 2863.4617 | 2863.4637 | -0.69 | 1 | 1 | 3.6 | 1Score > 19 indicates identity |
STLMKIIAGITPADSGTLEIEGNNYVR + Deamidated (NQ) | |
| 661.8649 | 1321.7152 | 1321.7129 | 1.76 | 0 | 1 | 2 | 1Score > 16 indicates identity |
LISLDTFSQLGK + Deamidated (NQ) | |
| 827.2371 | 4131.1491 | 4131.1774 | -6.83 | 1 | 1 | 1.8 | 1Score > 16 indicates identity |
LVGVTGTNGKTTTTQLLAQWSQLLGEISAVMGTVGNGLLGK + 2 Deamidated (NQ); Oxidation (M) | |
| 510.2500 | 2036.9709 | 2036.9836 | -6.26 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
APATQEEISEYNKLMASR | |
| 681.3671 | 2041.0795 | 2041.1109 | -15.4 | 0 | 1 | 3.1 | 1Score > 18 indicates identity |
IIGNIHNLQPWLPQELR + Deamidated (NQ) | |
| 420.8835 | 1259.6287 | 1259.6180 | 8.50 | 0 | 1 | 3.7 | 1Score > 19 indicates identity |
MQTTQQNAPLK + Deamidated (NQ) | |
| 544.6058 | 1630.7956 | 1630.8025 | -4.23 | 1 | 1 | 3.3 | 1Score > 18 indicates identity |
YGLIGANGSGKSTFMK + Deamidated (NQ) | |
| 476.5709 | 1426.6909 | 1426.6776 | 9.34 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
HCVWEEVARNK | |
| 1036.0422 | 4140.1397 | 4140.1194 | 4.90 | 1 | 1 | 1.6 | 1Score > 15 indicates identity |
FQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLK + 3 Deamidated (NQ) | |
| 467.2313 | 1864.8961 | 1864.9241 | -15.0 | 1 | 1 | 3.8 | 1Score > 19 indicates identity |
DLVSVGAYAKGSDPMLDK | |
| 509.9997 | 2035.9697 | 2035.9884 | -9.20 | 0 | 1 | 4.4 | 1Score > 20 indicates identity |
QDFLNSLTVEMLNQDLR + Deamidated (NQ) | |
| 484.2131 | 966.4116 | 966.4043 | 7.65 | 0 | 1 | 1.1 | 1Score > 13 indicates identity |
EGAYGNAER + Deamidated (NQ) | |
| 446.9033 | 1337.6881 | 1337.6683 | 14.8 | 1 | 1 | 3.6 | 1Score > 19 indicates identity |
LITREMVDSMK + Oxidation (M) | |
| 1131.5764 | 3391.7074 | 3391.7008 | 1.94 | 1 | 1 | 3.4 | 1Score > 18 indicates identity |
LGNTLGSDGENLPKAHIVATNPPFGSAAGTNITR + 2 Deamidated (NQ) | |
| 725.3665 | 1448.7184 | 1448.7293 | -7.50 | 1 | 1 | 4.2 | 1Score > 19 indicates identity |
TTQTLKMVAENGR + Deamidated (NQ) | |
| 624.3330 | 2493.3029 | 2493.2598 | 17.3 | 1 | 1 | 2.7 | 1Score > 17 indicates identity |
SEQLEQQLQVLLLPKDPDDER + Deamidated (NQ) | |
| 1015.5032 | 3043.4878 | 3043.5363 | -15.9 | 1 | 0 | 3.9 | 1Score > 19 indicates identity |
AYSVGGPGTPVLGPGGQFGATASAAGQRIAEAK + Deamidated (NQ) | |
| 672.3359 | 2013.9859 | 2013.9578 | 13.9 | 1 | 0 | 3.8 | 1Score > 19 indicates identity |
QQFRQQTGMTINQYLR + 3 Deamidated (NQ) | |
| 724.4293 | 2170.2661 | 2170.2296 | 16.8 | 1 | 0 | 0.9 | 1Score > 13 indicates identity |
MTHIVRFIGLLLLNASSLR + Deamidated (NQ); Oxidation (M) | |
| 1030.5277 | 2059.0408 | 2059.0639 | -11.2 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
WLFSFAGAPRPDNPKSIR + Deamidated (NQ) | |
| 866.9440 | 1731.8734 | 1731.8436 | 17.2 | 0 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
IIADIIAWCSGNMPR + Oxidation (M) | |
| 667.3644 | 1999.0714 | 1999.1102 | -19.4 | 1 | 0 | 3.1 | 1Score > 18 indicates identity |
LLRQQITDPVLFTQAVR + 2 Deamidated (NQ) | |
| 848.0840 | 2541.2302 | 2541.2281 | 0.80 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
MVAENQPGHIDQIKQTNAGAVYR + 2 Deamidated (NQ) | |
| 1062.0105 | 4244.0129 | 4243.9494 | 15.0 | 1 | 0 | 2.3 | 1Score > 17 indicates identity |
STPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGR + 2 Oxidation (M) | |
| 541.3149 | 540.3076 | 540.3020 | 10.4 | 0 | 0 | 0.91 | 1Score > 13 indicates identity |
VTGHK | |
| 897.8106 | 2690.4100 | 2690.4465 | -13.6 | 1 | 0 | 2.2 | 1Score > 16 indicates identity |
VIQGFGGAALMSVNTALIRLIYPQR + 3 Deamidated (NQ) | |
| 1152.0651 | 2302.1156 | 2302.0722 | 18.9 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
FATEGMMQVLADEYQQRLR + Deamidated (NQ); Oxidation (M) | |
| 300.1767 | 897.5083 | 897.5032 | 5.66 | 1 | 0 | 1.2 | 1Score > 14 indicates identity |
ALGAPKDAR | |
| 617.9980 | 3701.9443 | 3701.8875 | 15.4 | 1 | 0 | 1.5 | 1Score > 15 indicates identity |
LKPQNNLPELISGWRGELMAEALHNLLQEYPQ + Deamidated (NQ) | |
| 725.3645 | 2173.0717 | 2173.0287 | 19.8 | 1 | 0 | 4.4 | 1Score > 19 indicates identity |
DLRQAQEILDTDHYGLER + 2 Deamidated (NQ) | |
| 735.9055 | 4409.3893 | 4409.3655 | 5.40 | 1 | 0 | 1.1 | 1Score > 13 indicates identity |
QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTK + 2 Deamidated (NQ); Oxidation (M) | |
| 827.2365 | 4131.1461 | 4131.1774 | -7.56 | 1 | 0 | 1.8 | 1Score > 16 indicates identity |
LVGVTGTNGKTTTTQLLAQWSQLLGEISAVMGTVGNGLLGK + 2 Deamidated (NQ); Oxidation (M) | |
| 859.9586 | 1717.9026 | 1717.9111 | -4.93 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
SVRVAVFTQGANAEAAK | |
| 819.3834 | 1636.7522 | 1636.7766 | -14.9 | 0 | 0 | 3.2 | 1Score > 18 indicates identity |
AFGELAMTPQSQALR + 2 Deamidated (NQ); Oxidation (M) | |
| 595.9488 | 1784.8246 | 1784.8441 | -11.0 | 1 | 0 | 3.1 | 1Score > 18 indicates identity |
LEAAAQRFGDGHLNER + 2 Deamidated (NQ) | |
| 753.7390 | 4516.3903 | 4516.4050 | -3.24 | 1 | 0 | 1.1 | 1Score > 13 indicates identity |
GMLGLPFGIADPNFKIQLPPFALSVAVMCLVLAMIIFLPQR + 3 Deamidated (NQ); Oxidation (M) | |
| 523.5963 | 1567.7671 | 1567.7882 | -13.5 | 1 | 0 | 3.5 | 1Score > 18 indicates identity |
IVWSYDSSGVQAKK + Deamidated (NQ) | |
| 703.6941 | 2108.0605 | 2108.0498 | 5.07 | 0 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
GLNNPDLDAAVGEDLAQQLR | |
| 1089.5321 | 2177.0496 | 2177.0852 | -16.3 | 1 | 0 | 4.2 | 1Score > 19 indicates identity |
QVQINNPGDVYTKALDATTK + 2 Deamidated (NQ) | |
| 534.2541 | 1599.7405 | 1599.7351 | 3.33 | 1 | 0 | 2.4 | 1Score > 17 indicates identity |
YRGQVNVPDMFTR + 2 Deamidated (NQ); Oxidation (M) | |
| 1147.5800 | 2293.1454 | 2293.1624 | -7.40 | 0 | 0 | 4.2 | 1Score > 19 indicates identity |
LNVIFSCGTGDIVDLSSLDLR | |
| 818.7829 | 2453.3269 | 2453.3060 | 8.50 | 1 | 0 | 1.9 | 1Score > 16 indicates identity |
NMQITKILNNNVVVVIDDQQR | |
| 433.5747 | 1297.7023 | 1297.6779 | 18.8 | 0 | 0 | 2.3 | 1Score > 16 indicates identity |
AIVALFDHQQR + Deamidated (NQ) | |
| 593.6187 | 1777.8343 | 1777.8206 | 7.68 | 0 | 0 | 4 | 1Score > 19 indicates identity |
AVSDSGVPCYFIHGNR | |
| 737.8809 | 1473.7472 | 1473.7728 | -17.4 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
QFSPHSFQQLKK | |
| 529.6110 | 1585.8112 | 1585.7808 | 19.1 | 1 | 0 | 4.2 | 1Score > 19 indicates identity |
ASQNRDPAATSVAAAR + Deamidated (NQ) | |
| 850.4344 | 1698.8542 | 1698.8325 | 12.8 | 1 | 0 | 3.7 | 1Score > 18 indicates identity |
EQNEPVAQVSQWKR + Deamidated (NQ) | |
| 652.7054 | 1955.0944 | 1955.1091 | -7.56 | 1 | 0 | 1.7 | 1Score > 15 indicates identity |
IEKPKGVIVQYGGQTPLK + Deamidated (NQ) |
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the select summary.