MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4337 714.9165 3569.5461
4338 893.4319 3569.6985
4339 893.4415 3569.7369
4340 893.9379 3571.7225
4341 894.1713 3572.6561
4342 894.6913 3574.7361
4343 894.7371 3574.9193
4344 895.1934 3576.7445
4345 717.3387 3581.6571
4346 717.3426 3581.6766
4347 717.9827 3584.8771
4348 718.1841 3585.8841
4349 898.4401 3589.7313
4350 1197.9528 3590.8366
4351 899.4095 3593.6089
4352 899.4113 3593.6161
4353 900.4723 3597.8601
4354 720.7455 3598.6911
4355 903.4801 3609.8913
4356 723.7388 3613.6576
4357 904.4235 3613.6649
4358 905.6965 3618.7569
4359 905.9515 3619.7769
4360 906.4913 3621.9361
4361 906.7381 3622.9233
4362 909.9163 3635.6361
4363 911.9883 3643.9241
4364 913.7585 3651.0049
4365 914.9688 3655.8461
4366 914.9705 3655.8529
4367 915.9758 3659.8741
4368 733.1826 3660.8766
4369 919.9676 3675.8413
4370 736.3755 3676.8411
4371 920.7434 3678.9445
4372 617.8275 3700.9213
4373 529.7109 3700.9254
4374 529.8522 3701.9145
4375 529.8542 3701.9285
4377 741.3972 3701.9496
4378 618.1632 3702.9355
4379 933.5095 3730.0089
4380 933.9417 3731.7377
4381 934.4748 3733.8701
4382 938.4584 3749.8045
4383 938.7646 3751.0293
4384 940.2579 3757.0025
4385 943.2452 3768.9517
4386 949.9283 3795.6841
4389 957.9354 3827.7125
4390 958.5146 3830.0293
4391 960.4408 3837.7341
4392 960.6867 3838.7177
4393 962.2444 3844.9485
4394 962.2466 3844.9573
4395 962.2501 3844.9713
4396 771.0177 3850.0521
4397 964.4355 3853.7129
4398 964.4451 3853.7513
4400 773.1456 3860.6916
4401 966.4287 3861.6857
4402 967.6851 3866.7113
4404 777.5920 3882.9236
4405 972.0046 3883.9893
4406 973.5018 3889.9781
4407 975.9282 3899.6837
4409 786.4120 3927.0236
4410 786.4164 3927.0456
4411 1002.0303 4004.0921
4412 1002.5374 4006.1205
4413 808.8335 4039.1311
4414 812.2338 4056.1326
4415 812.4352 4057.1396
4416 677.2000 4057.1563
4417 677.2015 4057.1653
4419 1016.5211 4062.0553
4420 1016.7767 4063.0777
4421 1031.2833 4121.1041
4425 827.2384 4131.1556
4426 1035.5059 4137.9945
4428 828.8352 4139.1396
4429 828.8361 4139.1441
4431 1037.7924 4147.1405
4432 830.4417 4147.1721
4433 1038.0399 4148.1305
4434 830.6389 4148.1581
4435 830.6390 4148.1586
4436 1038.0479 4148.1625
4437 830.6431 4148.1791
4438 692.3713 4148.1841
4439 692.3737 4148.1985
4440 692.3738 4148.1991
4441 831.8336 4154.1316
4442 832.2325 4156.1261
4443 1041.2946 4161.1493
4444 833.4434 4162.1806
4445 833.6362 4163.1446
4447 835.4316 4172.1216
4448 1044.0505 4172.1729
4449 697.3728 4178.1931
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