MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4228 1135.2140 3402.6202
4230 852.1631 3404.6233
4231 1135.8826 3404.6260
4234 852.4070 3405.5989
4235 682.9219 3409.5731
4236 853.4040 3409.5869
4237 853.6541 3410.5873
4238 853.8997 3411.5697
4239 854.9130 3415.6229
4240 1139.8827 3416.6263
4241 1139.8835 3416.6287
4242 855.1659 3416.6345
4243 1140.8812 3419.6218
4244 855.9137 3419.6257
4245 1140.8832 3419.6278
4246 855.9158 3419.6341
4247 685.9160 3424.5436
4248 857.1667 3424.6377
4249 1142.8522 3425.5348
4250 857.4079 3425.6025
4251 857.4138 3425.6261
4252 1142.8833 3425.6281
4253 857.4151 3425.6313
4254 857.6425 3426.5409
4255 686.3174 3426.5506
4257 1144.5983 3430.7731
4258 1144.9390 3431.7952
4260 1145.2152 3432.6238
4261 861.3875 3441.5209
4262 861.3925 3441.5409
4263 861.4044 3441.5885
4264 861.6503 3442.5721
4265 689.5224 3442.5756
4266 689.5401 3442.6641
4267 862.4310 3445.6949
4269 863.9270 3451.6789
4270 865.3899 3457.5305
4271 865.4144 3457.6285
4272 865.4379 3457.7225
4273 692.7239 3458.5831
4274 869.3937 3473.5457
4275 871.1923 3480.7401
4276 871.9373 3483.7201
4279 877.6560 3506.5949
4280 877.6572 3506.5997
4282 1170.2053 3507.5941
4283 1170.2086 3507.6040
4284 878.6897 3510.7297
4285 878.9497 3511.7697
4286 879.6594 3514.6085
4287 1172.5465 3514.6177
4288 879.9140 3515.6269
4289 879.9141 3515.6273
4290 1172.8834 3515.6284
4291 879.9150 3515.6309
4292 880.4128 3517.6221
4293 881.9023 3523.5801
4294 1175.5378 3523.5916
4295 883.1909 3528.7345
4296 883.1934 3528.7445
4297 883.4084 3529.6045
4298 1177.5883 3529.7431
4299 883.6688 3530.6461
4300 883.9429 3531.7425
4301 884.1606 3532.6133
4302 707.5576 3532.7516
4303 884.4067 3533.5977
4304 1179.5427 3535.6063
4305 1180.8652 3539.5738
4306 1181.8701 3542.5885
4307 886.9371 3543.7193
4308 886.9378 3543.7221
4309 709.9172 3544.5496
4310 887.1830 3544.7029
4311 887.1929 3544.7425
4312 709.9579 3544.7531
4313 887.3892 3545.5277
4314 887.4001 3545.5713
4315 887.4142 3545.6277
4316 1182.8940 3545.6602
4317 1182.9119 3545.7139
4318 887.6657 3546.6337
4319 889.3994 3553.5685
4320 1185.5577 3553.6513
4321 711.9238 3554.5826
4322 889.9429 3555.7425
4323 890.2214 3556.8565
4324 890.2220 3556.8589
4325 890.4396 3557.7293
4326 890.9382 3559.7237
4327 890.9389 3559.7265
4328 890.9401 3559.7313
4329 891.1337 3560.5057
4330 891.1940 3560.7469
4331 713.3113 3561.5201
4332 892.6759 3566.6745
4333 714.3466 3566.6966
4334 892.9304 3567.6925
4335 892.9327 3567.7017
4336 893.3881 3569.5233
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