MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3989)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4112 1008.8616 3023.5630
4113 1008.8647 3023.5723
4114 606.5203 3027.5651
4115 606.5217 3027.5721
4116 606.5226 3027.5766
4117 1010.5535 3028.6387
4118 1010.5562 3028.6468
4119 1010.5562 3028.6468
4121 1015.8680 3044.5822
4122 1015.8689 3044.5849
4123 1017.8772 3050.6098
4126 1021.8713 3062.5921
4127 1023.2001 3066.5785
4128 767.6526 3066.5813
4129 1024.2340 3069.6802
4130 771.3946 3081.5493
4131 1028.8157 3083.4253
4132 1032.1312 3093.3718
4133 1032.5481 3094.6225
4134 1033.2128 3096.6166
4135 776.4178 3101.6421
4136 776.4184 3101.6445
4137 622.5411 3107.6691
4138 518.9522 3107.6695
4139 622.5414 3107.6706
4140 518.9526 3107.6719
4141 1037.4672 3109.3798
4142 1038.5492 3112.6258
4143 1044.5468 3130.6186
4144 1047.2179 3138.6319
4145 1047.5487 3139.6243
4146 785.9210 3139.6549
4147 1047.8380 3140.4922
4149 1053.4991 3157.4755
4150 1055.8412 3164.5018
4151 792.4184 3165.6445
4152 792.4198 3165.6501
4153 792.4208 3165.6541
4154 792.6668 3166.6381
4155 792.6708 3166.6541
4156 1057.6093 3169.8061
4157 1061.5214 3181.5424
4158 1061.5237 3181.5493
4159 1061.5240 3181.5502
4160 637.5067 3182.4971
4161 1065.1719 3192.4939
4162 802.4211 3205.6553
4163 1070.5610 3208.6612
4164 1070.8766 3209.6080
4165 1070.8787 3209.6143
4166 803.6604 3210.6125
4167 803.6621 3210.6193
4168 1074.5524 3220.6354
4169 806.6463 3222.5561
4170 1075.5314 3223.5724
4171 1075.5323 3223.5751
4172 1076.5427 3226.6063
4173 1079.8857 3236.6353
4174 1079.8860 3236.6362
4175 810.4185 3237.6449
4176 1082.5338 3244.5796
4177 813.7016 3250.7773
4178 814.4213 3253.6561
4179 1087.5270 3259.5592
4180 1087.5482 3259.6228
4181 1090.5023 3268.4851
4182 1091.5549 3271.6429
4183 819.6558 3274.5941
4184 546.8091 3274.8109
4185 657.5578 3282.7526
4189 826.1551 3300.5913
4191 1101.5400 3301.5982
4193 1101.9305 3302.7697
4195 829.6683 3314.6441
4196 830.3998 3317.5701
4197 1109.2167 3324.6283
4198 1110.5372 3328.5898
4199 1110.5411 3328.6015
4200 833.1625 3328.6209
4201 1110.5507 3328.6303
4202 833.1649 3328.6305
4203 833.4035 3329.5849
4204 1111.2007 3330.5803
4205 833.6823 3330.7001
4206 1112.5951 3334.7635
4207 669.3607 3341.7671
4208 839.4150 3353.6309
4214 844.4182 3373.6437
4215 844.4258 3373.6741
4216 844.4480 3373.7629
4217 845.1429 3376.5425
4218 845.4142 3377.6277
4219 1128.5714 3382.6924
4220 1130.2139 3387.6199
4221 847.9125 3387.6209
4222 847.9171 3387.6393
4223 1130.5509 3388.6309
4224 1130.5549 3388.6429
4225 848.4164 3389.6365
4227 849.6587 3394.6057
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