MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3993 923.1719 2766.4939
3994 923.4586 2767.3540
3995 555.1000 2770.4636
3996 693.6240 2770.4669
3997 693.6244 2770.4685
3998 693.6254 2770.4725
3999 555.1019 2770.4731
4000 693.6259 2770.4745
4001 555.1022 2770.4746
4002 924.4996 2770.4770
4003 555.1044 2770.4856
4004 925.1694 2772.4864
4005 925.5071 2773.4995
4006 925.8286 2774.4640
4007 925.8372 2774.4898
4008 925.8375 2774.4907
4009 926.5022 2776.4848
4010 927.0932 2778.2578
4011 928.1445 2781.4117
4012 929.4571 2785.3495
4013 929.4625 2785.3657
4014 929.5034 2785.4884
4015 698.1198 2788.4501
4016 558.6973 2788.4501
4017 698.1216 2788.4573
4019 931.1875 2790.5407
4020 932.4417 2794.3033
4022 933.0994 2796.2764
4024 701.3405 2801.3329
4026 934.8353 2801.4841
4027 701.8632 2803.4237
4028 936.8477 2807.5213
4029 702.8893 2807.5281
4030 702.8902 2807.5317
4031 703.1381 2808.5233
4032 703.8658 2811.4341
4033 938.5037 2812.4893
4034 941.1275 2820.3607
4035 942.1182 2823.3328
4036 942.1188 2823.3346
4037 942.1213 2823.3421
4038 942.1224 2823.3454
4039 942.4532 2824.3378
4042 947.4509 2839.3309
4043 947.4530 2839.3372
4044 947.7917 2840.3533
4045 948.8787 2843.6143
4046 949.1321 2844.3745
4047 949.4494 2845.3264
4048 949.4604 2845.3594
4049 950.8375 2849.4907
4051 713.6489 2850.5665
4052 953.5421 2857.6045
4053 716.3351 2861.3113
4054 954.8049 2861.3929
4055 955.4727 2863.3963
4058 955.4977 2863.4713
4059 955.5355 2863.5847
4060 956.4774 2866.4104
4062 718.3730 2869.4629
4063 959.8308 2876.4706
4064 959.8311 2876.4715
4065 960.4894 2878.4464
4066 720.8704 2879.4525
4068 720.8742 2879.4677
4069 961.8163 2882.4271
4070 963.2040 2886.5902
4071 965.1814 2892.5224
4072 724.8664 2895.4365
4073 724.8686 2895.4453
4074 966.1754 2895.5044
4075 967.8442 2900.5108
4076 727.8721 2907.4593
4077 970.5183 2908.5331
4078 728.8722 2911.4597
4083 972.5082 2914.5028
4084 973.8596 2918.5570
4085 973.8608 2918.5606
4086 977.8628 2930.5666
4087 735.3779 2937.4825
4088 735.3922 2937.5397
4089 736.3970 2941.5589
4090 982.4721 2944.3945
4091 982.4748 2944.4026
4092 982.4755 2944.4047
4093 982.8112 2945.4118
4094 738.6376 2950.5213
4097 591.3207 2951.5671
4099 990.5195 2968.5367
4100 990.5394 2968.5964
4101 991.7902 2972.3488
4102 748.6861 2990.7153
4103 998.4813 2992.4221
4104 998.8231 2993.4475
4105 752.9049 3007.5905
4106 753.9069 3011.5985
4108 1005.2084 3012.6034
4109 1006.2280 3015.6622
4110 755.6313 3018.4961
4111 1008.2205 3021.6397
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