MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3887 905.4885 2713.4437
3888 543.6966 2713.4466
3889 679.3690 2713.4469
3890 905.4902 2713.4488
3891 679.3695 2713.4489
3893 543.6974 2713.4506
3894 543.6982 2713.4546
3895 679.3710 2713.4549
3896 679.3712 2713.4557
3897 543.6986 2713.4566
3898 543.6988 2713.4576
3899 679.3718 2713.4581
3900 543.6989 2713.4581
3901 543.6992 2713.4596
3902 679.3723 2713.4601
3903 543.6994 2713.4606
3904 679.3726 2713.4613
3905 679.3727 2713.4617
3906 543.6998 2713.4626
3907 543.6998 2713.4626
3908 543.7001 2713.4641
3909 679.3734 2713.4645
3910 543.7004 2713.4656
3911 679.3738 2713.4661
3912 543.7006 2713.4666
3913 679.3789 2713.4865
3914 905.8293 2714.4661
3915 906.4333 2716.2781
3916 680.3455 2717.3529
3917 680.6127 2718.4217
3918 681.1129 2720.4225
3919 907.8188 2720.4346
3922 908.8011 2723.3815
3923 908.8022 2723.3848
3924 908.8028 2723.3866
3925 682.7753 2727.0721
3926 910.1194 2727.3364
3927 910.1232 2727.3478
3928 683.1189 2728.4465
3929 683.1209 2728.4545
3930 910.8184 2729.4334
3931 683.3661 2729.4353
3932 683.3662 2729.4357
3933 546.8945 2729.4361
3934 546.8948 2729.4376
3935 683.3672 2729.4397
3936 683.3676 2729.4413
3937 546.8957 2729.4421
3938 910.8215 2729.4427
3939 683.3696 2729.4493
3940 546.8972 2729.4496
3941 546.8992 2729.4596
3942 546.8996 2729.4616
3943 683.5693 2730.2481
3944 683.5713 2730.2561
3947 912.4666 2734.3780
3948 913.1439 2736.4099
3949 685.1106 2736.4133
3950 548.4890 2737.4086
3951 685.6249 2738.4705
3952 548.9016 2739.4716
3953 685.8754 2739.4725
3954 548.9023 2739.4751
3955 685.8778 2739.4821
3956 549.0950 2740.4386
3957 686.3658 2741.4341
3958 686.3667 2741.4377
3959 549.2955 2741.4411
3960 686.3712 2741.4557
3961 686.3716 2741.4573
3962 686.6240 2742.4669
3963 687.3666 2745.4373
3964 687.6163 2746.4361
3965 916.4867 2746.4383
3966 687.6180 2746.4429
3968 917.8420 2750.5042
3969 917.8429 2750.5069
3970 688.6340 2750.5069
3971 688.6343 2750.5081
3972 551.2839 2751.3831
3973 551.2859 2751.3931
3974 918.1724 2751.4954
3975 918.4715 2752.3927
3976 689.1082 2752.4037
3977 689.6259 2754.4745
3978 551.9027 2754.4771
3979 919.4717 2755.3933
3980 689.8746 2755.4693
3981 690.3629 2757.4225
3982 690.3751 2757.4713
3983 691.3660 2761.4349
3984 691.6157 2762.4337
3985 922.1320 2763.3742
3986 922.1328 2763.3766
3987 922.1344 2763.3814
3988 922.8342 2765.4808
3989 692.5947 2766.3497
3990 554.2781 2766.3541
3991 692.5973 2766.3601
3992 554.2829 2766.3781
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