MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3766 856.4406 2566.3000
3767 857.0976 2568.2710
3768 857.7350 2570.1832
3769 857.7386 2570.1940
3770 857.7552 2570.2438
3772 860.4164 2578.2274
3773 861.1006 2580.2800
3774 861.4279 2581.2619
3775 861.4482 2581.3228
3776 862.4477 2584.3213
3778 863.4091 2587.2055
3779 864.4910 2590.4512
3780 651.0958 2600.3541
3781 651.0961 2600.3553
3782 867.7928 2600.3566
3783 869.4092 2605.2058
3784 870.1407 2607.4003
3785 871.0700 2610.1882
3786 873.0794 2616.2164
3787 873.0810 2616.2212
3788 873.0832 2616.2278
3789 873.0905 2616.2497
3790 874.0713 2619.1921
3791 656.5999 2622.3705
3792 657.2943 2625.1481
3793 876.3884 2626.1434
3794 657.8476 2627.3613
3795 878.4151 2632.2235
3796 878.4173 2632.2301
3797 878.4523 2632.3351
3798 878.4534 2632.3384
3799 878.7848 2633.3326
3801 879.7363 2636.1871
3805 880.0779 2637.2119
3806 880.0835 2637.2287
3807 660.8441 2639.3473
3808 881.7031 2642.0875
3809 661.5389 2642.1265
3810 883.4681 2647.3825
3811 883.4727 2647.3963
3812 883.4738 2647.3996
3813 884.4111 2650.2115
3814 885.0718 2652.1936
3815 885.0726 2652.1960
3816 885.0793 2652.2161
3818 885.4005 2653.1797
3819 885.4039 2653.1899
3820 885.4549 2653.3429
3821 885.4612 2653.3618
3822 532.2812 2656.3696
3823 665.1002 2656.3717
3824 887.0685 2658.1837
3825 665.8020 2659.1789
3827 888.4172 2662.2298
3828 888.4178 2662.2316
3829 889.0729 2664.1969
3830 890.1605 2667.4597
3831 890.4959 2668.4659
3834 669.5441 2674.1473
3835 892.3909 2674.1509
3836 669.5479 2674.1625
3837 892.7255 2675.1547
3839 893.4155 2677.2247
3842 895.7861 2684.3365
3843 672.0945 2684.3489
3846 896.4683 2686.3831
3847 897.0978 2688.2716
3848 897.3787 2689.1143
3849 897.4233 2689.2481
3850 897.4316 2689.2730
3851 897.4329 2689.2769
3852 673.5382 2690.1237
3853 897.7175 2690.1307
3854 673.5411 2690.1353
3856 673.6130 2690.4229
3857 673.8591 2691.4073
3858 673.8619 2691.4185
3859 898.4421 2692.3045
3860 898.4426 2692.3060
3861 898.4432 2692.3078
3862 898.4471 2692.3195
3864 898.7852 2693.3338
3865 899.0846 2694.2320
3867 675.0381 2696.1233
3868 540.2931 2696.4291
3870 540.6912 2698.4196
3871 676.6346 2702.5093
3872 676.8600 2703.4109
3873 902.1450 2703.4132
3874 676.8609 2703.4145
3875 902.4282 2704.2628
3876 677.5353 2706.1121
3878 903.8110 2708.4112
3879 679.0305 2712.0929
3880 543.4272 2712.0996
3882 543.6944 2713.4356
3883 679.3668 2713.4381
3884 905.4868 2713.4386
3885 679.3673 2713.4401
3886 543.6954 2713.4406
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