MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3293 492.2710 1965.0549
3294 492.2733 1965.0641
3295 492.5190 1966.0469
3296 492.5201 1966.0513
3297 493.0224 1968.0605
3298 658.0404 1971.0994
3299 494.0159 1972.0345
3300 659.0240 1974.0502
3302 659.3657 1975.0753
3303 496.0081 1980.0033
3304 661.0371 1980.0895
3309 993.0022 1983.9898
3323 662.3677 1984.0813
3324 497.7673 1987.0401
3326 663.6897 1988.0473
3327 664.0214 1989.0424
3329 667.3561 1999.0465
3330 667.3593 1999.0561
3332 667.6960 2000.0662
3333 401.2887 2001.4071
3334 1002.2784 2002.5422
3335 669.0330 2004.0772
3338 669.6910 2006.0512
3339 502.7629 2007.0225
3342 1005.5514 2009.0882
3343 671.0393 2010.0961
3344 503.7712 2011.0557
3346 504.0215 2012.0569
3349 1007.4611 2012.9076
3352 673.0245 2016.0517
3353 673.3633 2017.0681
3357 405.2082 2021.0046
3358 506.2589 2021.0065
3359 405.2086 2021.0066
3361 506.5081 2022.0033
3364 675.0374 2022.0904
3365 675.4014 2023.1824
3366 675.4038 2023.1896
3367 678.3356 2031.9850
3371 679.9985 2036.9737
3372 680.3647 2038.0723
3373 680.6876 2039.0410
3374 681.0322 2040.0748
3376 511.2775 2041.0809
3378 1026.0640 2050.1134
3379 684.3805 2050.1197
3387 1031.5370 2061.0594
3388 689.0107 2064.0103
3392 518.7664 2071.0365
3393 518.7667 2071.0377
3395 691.3542 2071.0408
3397 518.7697 2071.0497
3398 691.6475 2071.9207
3399 1037.5431 2073.0716
3400 1037.5469 2073.0792
3401 692.0342 2073.0808
3402 1038.0442 2074.0738
3403 692.3691 2074.0855
3404 692.3785 2074.1137
3405 692.3785 2074.1137
3406 692.3790 2074.1152
3407 692.3813 2074.1221
3408 519.7362 2074.9157
3409 692.6854 2075.0344
3410 692.7130 2075.1172
3411 1039.5389 2077.0632
3412 1041.0463 2080.0780
3414 695.7105 2084.1097
3415 695.7166 2084.1280
3418 522.7655 2087.0329
3419 522.7656 2087.0333
3420 699.0583 2094.1531
3422 700.0005 2096.9797
3423 700.0020 2096.9842
3428 526.0384 2100.1245
3429 421.0326 2100.1266
3430 703.3611 2107.0615
3431 703.6785 2108.0137
3433 1055.4983 2108.9820
3434 704.9899 2111.9479
3436 529.5136 2114.0253
3437 529.7652 2115.0317
3438 1058.5352 2115.0558
3440 1058.5796 2115.1446
3441 706.0569 2115.1489
3442 529.7982 2115.1637
3444 706.7028 2117.0866
3445 706.7344 2117.1814
3446 707.7099 2120.1079
3451 711.0276 2130.0610
3452 1071.5619 2141.1092
3453 714.7239 2141.1499
3454 717.0309 2148.0709
3455 538.0256 2148.0733
3456 717.0568 2148.1486
3457 718.4144 2152.2214
3458 718.7466 2153.2180
3462 1080.5640 2159.1134
3463 1081.0663 2160.1180
3466 721.3792 2161.1158
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