MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2438 |
582.2924 | 1162.5702 | 1162.5870 | -14.4 | 0 | 8 | 0.61 | 1Score > 19 indicates identity |
NGVNVSFSIPK + 2 Deamidated (NQ) |
| 1997 |
449.7626 | 897.5106 | 897.5144 | -4.20 | 1 | 8 | 0.17 | 1Score > 13 indicates identity |
LAGSPRAAR |
| 3084 |
860.4208 | 1718.8270 | 1718.8151 | 6.93 | 1 | 8 | 0.8 | 1Score > 20 indicates identity |
NYVVKLQSGDEYFR + 2 Deamidated (NQ) |
| 1912 |
822.4178 | 821.4105 | 821.4031 | 8.99 | 0 | 8 | 0.34 | 1Score > 16 indicates identity |
AFNSLNR + Deamidated (NQ) |
| 1547 |
515.3312 | 514.3239 | 514.3227 | 2.34 | 0 | 8 | 0.71 | 1Score > 22 indicates identity Score > 19 indicates homology |
LAGVR |
| 2063 |
473.2743 | 944.5340 | 944.5291 | 5.27 | 0 | 8 | 0.65 | 1Score > 19 indicates identity |
LALNVASTR + Deamidated (NQ) |
| 1875 |
401.6959 | 801.3772 | 801.3803 | -3.82 | 0 | 8 | 0.15 | 1Score > 13 indicates identity |
MTHQLR + Deamidated (NQ); Oxidation (M) |
| 2519 |
612.3494 | 1222.6842 | 1222.6809 | 2.77 | 1 | 8 | 0.25 | 1Score > 15 indicates identity |
IEIYSKTLEK |
| 3439 |
706.0266 | 2115.0580 | 2115.0882 | -14.3 | 1 | 8 | 0.69 | 1Score > 19 indicates identity |
MDSVPVIVLSGLTDEQKQR + Deamidated (NQ) |
| 2777 |
731.3661 | 1460.7176 | 1460.7372 | -13.4 | 1 | 8 | 0.51 | 1Score > 20 indicates identity Score > 18 indicates homology |
WQSTSVNDVLRR + Deamidated (NQ) |
| 2104 |
482.7326 | 963.4506 | 963.4661 | -16.1 | 0 | 8 | 0.5 | 1Score > 18 indicates identity |
FQEQVNAK + Deamidated (NQ) |
| 2110 |
483.2619 | 964.5092 | 964.4978 | 11.9 | 1 | 8 | 0.63 | 1Score > 19 indicates identity |
QQYREIK + Deamidated (NQ) |
| 2015 |
457.7524 | 913.4902 | 913.4730 | 18.9 | 0 | 8 | 0.32 | 1Score > 16 indicates identity |
GANQAVAAGR |
| 3503 |
743.0306 | 2226.0700 | 2226.0335 | 16.4 | 0 | 8 | 0.74 | 1Score > 19 indicates identity |
IIEQMEAQGIVSEQGHNGNR + Deamidated (NQ); Oxidation (M) |
| 2240 |
517.7861 | 1033.5576 | 1033.5556 | 1.95 | 0 | 8 | 0.57 | 1Score > 18 indicates identity |
LAQGEFLTR |
| 2242 |
517.7878 | 1033.5610 | 1033.5556 | 5.24 | 0 | 8 | 0.61 | 1Score > 18 indicates identity |
LAQGEFLTR |
| 2306 |
364.8751 | 1091.6035 | 1091.5975 | 5.50 | 1 | 8 | 0.51 | 1Score > 17 indicates identity |
DNVLRYIAK + Deamidated (NQ) |
| 2441 |
389.1915 | 1164.5527 | 1164.5636 | -9.34 | 1 | 8 | 0.7 | 1Score > 19 indicates identity |
QEYTGARNAR |
| 1755 |
697.4272 | 696.4199 | 696.4283 | -12.0 | 0 | 8 | 0.17 | 1Score > 13 indicates identity |
LPNVVR |
| 2338 |
557.8119 | 1113.6092 | 1113.6142 | -4.44 | 1 | 8 | 0.67 | 1Score > 18 indicates identity |
NKSPVEATLR |
| 3196 |
928.4531 | 1854.8916 | 1854.9264 | -18.7 | 1 | 8 | 0.84 | 1Score > 19 indicates identity |
TLAAFWYLKSAQQGNR + 2 Deamidated (NQ) |
| 1752 |
346.2130 | 690.4114 | 690.4024 | 13.1 | 1 | 7 | 0.21 | 1Score > 13 indicates identity |
TKSSLR |
| 1839 |
382.1936 | 762.3726 | 762.3582 | 19.0 | 0 | 7 | 0.62 | 1Score > 18 indicates identity |
LEQDMK |
| 2239 |
515.7899 | 1029.5652 | 1029.5567 | 8.29 | 0 | 7 | 0.24 | 1Score > 21 indicates identity Score > 14 indicates homology |
VGVTGNISQR |
| 2352 |
560.8032 | 1119.5918 | 1119.6036 | -10.5 | 0 | 7 | 0.7 | 1Score > 18 indicates identity |
HPELLNEIR |
| 2649 |
687.3591 | 1372.7036 | 1372.7198 | -11.8 | 0 | 7 | 0.86 | 1Score > 19 indicates identity |
SQAAADLLAVTDAK |
| 2157 |
492.7866 | 983.5586 | 983.5763 | -18.0 | 1 | 7 | 0.34 | 1Score > 15 indicates identity |
LPKAEIASR |
| 2291 |
535.2759 | 1068.5372 | 1068.5240 | 12.4 | 0 | 7 | 0.49 | 1Score > 17 indicates identity |
VLADGQFYR + Deamidated (NQ) |
| 4041 |
946.4783 | 2836.4131 | 2836.3742 | 13.7 | 0 | 7 | 1 | 1Score > 20 indicates identity Score > 20 indicates homology |
NLTDYGAFVDLGGVDGLLHITDMAWK + Oxidation (M) |
| 2007 |
455.2639 | 908.5132 | 908.4967 | 18.2 | 0 | 7 | 0.28 | 1Score > 14 indicates identity |
IAVSYQTK |
| 1696 |
664.3058 | 663.2985 | 663.3010 | -3.73 | 0 | 7 | 0.38 | 1Score > 16 indicates identity |
LCQSR + Deamidated (NQ) |
| 2433 |
581.3307 | 1160.6468 | 1160.6475 | -0.54 | 1 | 7 | 0.59 | 1Score > 17 indicates identity |
GVQIMKEIVK + Deamidated (NQ); Oxidation (M) |
| 2451 |
392.8722 | 1175.5948 | 1175.6160 | -18.0 | 1 | 7 | 0.76 | 1Score > 18 indicates identity |
LGRDGATPHPR |
| 2221 |
508.7833 | 1015.5520 | 1015.5662 | -13.9 | 0 | 7 | 0.94 | 1Score > 19 indicates identity |
LESQIGTLR |
| 1750 |
346.1761 | 690.3376 | 690.3483 | -15.4 | 1 | 7 | 0.54 | 1Score > 17 indicates identity |
KCNLR + Deamidated (NQ) |
| 1695 |
332.6564 | 663.2982 | 663.3010 | -4.16 | 0 | 7 | 0.39 | 1Score > 16 indicates identity |
GDGLMR + Oxidation (M) |
| 1979 |
437.7422 | 873.4698 | 873.4556 | 16.4 | 0 | 7 | 0.32 | 1Score > 21 indicates identity Score > 15 indicates homology |
DIINAATR + Deamidated (NQ) |
| 4408 |
979.9327 | 3915.7017 | 3915.7269 | -6.42 | 1 | 7 | 0.25 | 1Score > 13 indicates identity |
YDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGK + 4 Deamidated (NQ); Oxidation (M) |
| 3599 |
586.0222 | 2340.0597 | 2340.0943 | -14.8 | 0 | 7 | 0.53 | 1Score > 17 indicates identity |
YLNNINGLLTSFCGADAPQLR + 4 Deamidated (NQ) |
| 2538 |
412.5488 | 1234.6246 | 1234.6305 | -4.83 | 1 | 7 | 0.79 | 1Score > 18 indicates identity |
IANYLERQAR + 2 Deamidated (NQ) |
| 2503 |
404.2189 | 1209.6349 | 1209.6393 | -3.68 | 0 | 7 | 0.47 | 1Score > 16 indicates identity |
NYQAALFILR + 2 Deamidated (NQ) |
| 3347 |
671.6951 | 2012.0635 | 2012.0261 | 18.6 | 1 | 7 | 0.66 | 1Score > 18 indicates identity |
IRQMPGLNAIEHSNYLR + Deamidated (NQ) |
| 1693 |
329.2245 | 656.4344 | 656.4333 | 1.72 | 1 | 7 | 0.23 | 1Score > 13 indicates identity |
AVIKAR |
| 1500 |
489.2788 | 488.2715 | 488.2707 | 1.74 | 0 | 7 | 0.86 | 1Score > 20 indicates identity Score > 19 indicates homology |
TAAAR |
| 4629 |
1028.9403 | 5139.6651 | 5139.6220 | 8.39 | 1 | 7 | 0.28 | 1Score > 14 indicates identity |
GRLMQALPAGGAMVAVAASQHEVEPLLVEGVDIAALNAPGSVVISGDQAAVR + 3 Deamidated (NQ); 2 Oxidation (M) |
| 2555 |
417.8829 | 1250.6269 | 1250.6367 | -7.86 | 0 | 7 | 0.91 | 1Score > 19 indicates identity |
ANQLAELAHQR + Deamidated (NQ) |
| 1628 |
607.2846 | 606.2773 | 606.2795 | -3.63 | 0 | 7 | 0.48 | 1Score > 16 indicates identity |
NSVMR + Deamidated (NQ) |
| 2379 |
568.7854 | 1135.5562 | 1135.5478 | 7.46 | 1 | 7 | 0.95 | 1Score > 19 indicates identity |
MSKICNDLR |
| 2194 |
500.7889 | 999.5632 | 999.5712 | -8.00 | 1 | 7 | 0.81 | 1Score > 18 indicates identity |
LALAKEEAR |
| 2348 |
373.8716 | 1118.5930 | 1118.6044 | -10.2 | 1 | 7 | 0.94 | 1Score > 19 indicates identity |
QTGRTLTSAGK |
| 2717 |
713.3825 | 1424.7504 | 1424.7459 | 3.20 | 1 | 7 | 0.57 | 1Score > 17 indicates identity |
WRHQAIQALMR + Oxidation (M) |
| 2077 |
475.2350 | 948.4554 | 948.4665 | -11.6 | 0 | 7 | 0.79 | 1Score > 18 indicates identity |
AEIDAGFAR |
| 2092 |
480.2774 | 958.5402 | 958.5487 | -8.86 | 0 | 7 | 0.83 | 1Score > 18 indicates identity |
IAQAPAFLK + Deamidated (NQ) |
| 2714 |
475.9207 | 1424.7403 | 1424.7234 | 11.8 | 1 | 7 | 0.67 | 1Score > 17 indicates identity |
VEIAPFAYMRGR + Oxidation (M) |
| 3643 |
797.4296 | 2389.2670 | 2389.2740 | -2.96 | 1 | 7 | 0.56 | 1Score > 17 indicates identity |
SSLPLGKFLVTEGVISQETLDR + Deamidated (NQ) |
| 1973 |
437.2122 | 872.4098 | 872.4141 | -4.83 | 0 | 7 | 0.33 | 1Score > 14 indicates identity |
WPGETQR |
| 3471 |
724.7259 | 2171.1559 | 2171.1222 | 15.5 | 1 | 7 | 0.57 | 1Score > 17 indicates identity |
LEGWSESGAQAKIAIAEGQVK |
| 1754 |
697.4257 | 696.4184 | 696.4283 | -14.1 | 0 | 7 | 0.22 | 1Score > 13 indicates identity |
LPNVVR |
| 2158 |
492.7906 | 983.5666 | 983.5763 | -9.85 | 1 | 6 | 0.31 | 1Score > 14 indicates identity |
ALAIAAREVA |
| 3356 |
674.6743 | 2021.0011 | 2020.9623 | 19.2 | 1 | 6 | 0.34 | 1Score > 20 indicates identity Score > 14 indicates homology |
AITGEEMTQEKLDLAAER + Deamidated (NQ); Oxidation (M) |
| 3866 |
674.8702 | 2695.4517 | 2695.4190 | 12.1 | 1 | 6 | 0.48 | 1Score > 16 indicates identity |
VFPESIVRTVVHMMESVALPGGGGVK |
| 1817 |
372.7235 | 743.4324 | 743.4289 | 4.72 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
ALARNAK + Deamidated (NQ) |
| 3601 |
586.0239 | 2340.0665 | 2340.0725 | -2.58 | 1 | 6 | 0.71 | 1Score > 17 indicates identity |
QAESMLESMASRIDNYPELR + Deamidated (NQ) |
| 1814 |
372.7218 | 743.4290 | 743.4289 | 0.15 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
ALARNAK + Deamidated (NQ) |
| 2268 |
350.8471 | 1049.5195 | 1049.5288 | -8.88 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
RMTGVTVDR + Oxidation (M) |
| 2399 |
572.3576 | 1142.7006 | 1142.6910 | 8.41 | 1 | 6 | 0.23 | 1Score > 13 indicates identity |
KQTALVELLK + Deamidated (NQ) |
| 2202 |
501.7920 | 1001.5694 | 1001.5692 | 0.28 | 1 | 6 | 0.96 | 1Score > 19 indicates identity |
LAPVMAKTR + Oxidation (M) |
| 2203 |
501.7929 | 1001.5712 | 1001.5869 | -15.7 | 0 | 6 | 0.53 | 1Score > 16 indicates identity |
SVGTSLLVAR |
| 2145 |
491.2508 | 980.4870 | 980.5039 | -17.2 | 1 | 6 | 0.77 | 1Score > 18 indicates identity |
QQRYSATK |
| 3946 |
911.8293 | 2732.4661 | 2732.4968 | -11.3 | 1 | 6 | 0.51 | 1Score > 16 indicates identity |
ALAAIGLAAVMSQSAMAENLKLGFLVK + Oxidation (M) |
| 2529 |
614.8386 | 1227.6626 | 1227.6571 | 4.52 | 1 | 6 | 1 | 1Score > 19 indicates identity |
QRLDAINQLR + 2 Deamidated (NQ) |
| 2122 |
485.2862 | 968.5578 | 968.5403 | 18.1 | 1 | 6 | 0.31 | 1Score > 14 indicates identity |
ARLQPQQK + Deamidated (NQ) |
| 2257 |
521.7994 | 1041.5842 | 1041.5679 | 15.7 | 1 | 6 | 0.77 | 1Score > 17 indicates identity |
LQQVAGRDR |
| 2006 |
455.2630 | 908.5114 | 908.4967 | 16.2 | 0 | 6 | 0.32 | 1Score > 14 indicates identity |
IAVSYQTK |
| 3465 |
721.3785 | 2161.1137 | 2161.0956 | 8.35 | 1 | 6 | 0.92 | 1Score > 18 indicates identity |
SHWATFKQTATNLWVTLR + 2 Deamidated (NQ) |
| 2193 |
500.7881 | 999.5616 | 999.5713 | -9.66 | 0 | 6 | 0.8 | 1Score > 18 indicates identity |
LGGGTVLQQK |
| 3325 |
663.3549 | 1987.0429 | 1987.0626 | -9.92 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
ELLTTLNLKLEPADDFR |
| 2422 |
578.3246 | 1154.6346 | 1154.6481 | -11.7 | 0 | 6 | 0.56 | 1Score > 16 indicates identity |
IGAIMVPINAR + Deamidated (NQ) |
| 1501 |
489.2795 | 488.2722 | 488.2707 | 3.17 | 0 | 6 | 0.85 | 1Score > 21 indicates identity Score > 18 indicates homology |
TAAAR |
| 2778 |
731.3685 | 1460.7224 | 1460.7372 | -10.1 | 1 | 6 | 0.3 | 1Score > 20 indicates identity Score > 13 indicates homology |
WQSTSVNDVLRR + Deamidated (NQ) |
| 3057 |
418.9310 | 1671.6949 | 1671.7246 | -17.8 | 1 | 6 | 0.26 | 1Score > 13 indicates identity |
MNFSHGSPEDHKMR |
| 3382 |
1030.5236 | 2059.0326 | 2059.0011 | 15.3 | 0 | 6 | 0.51 | 1Score > 20 indicates identity Score > 15 indicates homology |
NWLTADGAPGPTGTGGFIAEK |
| 3120 |
592.9709 | 1775.8909 | 1775.9240 | -18.7 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
FVVMPGRDTVVDVQSK |
| 2760 |
723.8889 | 1445.7632 | 1445.7548 | 5.87 | 0 | 6 | 0.91 | 1Score > 18 indicates identity |
MAIAEAITNIAATR + Deamidated (NQ) |
| 1868 |
400.7366 | 799.4586 | 799.4552 | 4.35 | 0 | 6 | 0.93 | 1Score > 18 indicates identity |
LAQAAGIR + Deamidated (NQ) |
| 2452 |
392.8724 | 1175.5954 | 1175.6160 | -17.5 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
LGRDGATPHPR |
| 3355 |
674.3680 | 2020.0822 | 2020.0953 | -6.49 | 0 | 6 | 0.62 | 1Score > 16 indicates identity |
LISVHNADLNVVLTTPASR + Deamidated (NQ) |
| 2500 |
605.8064 | 1209.5982 | 1209.5850 | 10.9 | 0 | 6 | 0.87 | 1Score > 17 indicates identity |
QQHSDVRPSR + Deamidated (NQ) |
| 4403 |
971.4843 | 3881.9081 | 3881.8366 | 18.4 | 0 | 6 | 0.92 | 1Score > 18 indicates identity |
LHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVK + Deamidated (NQ) |
| 1644 |
309.1685 | 616.3224 | 616.3180 | 7.20 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
EEAIR |
| 2036 |
465.7283 | 929.4420 | 929.4566 | -15.7 | 0 | 5 | 0.41 | 1Score > 14 indicates identity |
VDVAENQR |
| 3200 |
931.4991 | 1860.9836 | 1860.9945 | -5.82 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
INITLQKEEGGLYNLR + Deamidated (NQ) |
| 1598 |
574.3217 | 573.3144 | 573.3122 | 3.89 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
AENIK |
| 3425 |
525.2538 | 2096.9861 | 2096.9911 | -2.39 | 0 | 5 | 0.32 | 1Score > 20 indicates identity Score > 13 indicates homology |
VIPGFMIQGGGFTEQMQQK + 2 Deamidated (NQ) |
| 1577 |
559.3254 | 558.3181 | 558.3125 | 9.99 | 0 | 5 | 0.57 | 1Score > 22 indicates identity Score > 15 indicates homology |
AVNVR + Deamidated (NQ) |
| 1987 |
890.4249 | 889.4176 | 889.4042 | 15.1 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
FGGNPENR |
| 2686 |
703.3741 | 1404.7336 | 1404.7547 | -15.0 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
WNAIMTVLRASK + Oxidation (M) |
| 3427 |
525.7665 | 2099.0369 | 2099.0647 | -13.2 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
AIPNQGVQKQIDLFGNAER + 2 Deamidated (NQ) |
| 3754 |
850.7610 | 2549.2612 | 2549.2835 | -8.77 | 1 | 5 | 0.72 | 1Score > 20 indicates identity Score > 16 indicates homology |
GLMNVQFAVKNNEVYLIEVNPR + 3 Deamidated (NQ) |
| 2527 |
410.2266 | 1227.6580 | 1227.6571 | 0.71 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
QRLDAINQLR + 2 Deamidated (NQ) |
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