MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (63 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 RHAA_ECOLI 6577 47455 276 (235) 46 (43) 587.65 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2
2 PEPB_ECOLI 1615 46436 55 (50) 25 (23) 9.34 Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2
3 IADA_ECOLI 651 41400 21 (20) 13 (12) 2.17 Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1
4 CODA_ECOLI 463 47732 24 (19) 17 (14) 2.33 Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3
5 ALF1_ECOLI 333 38313 13 (13) 10 (10) 1.49 Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2
6 CATNterm_ECOLX 324 27452 14 (13) 7 (7) 2.15 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1
7 YGEY_ECOLI 280 45288 12 (11) 8 (8) 0.88 Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1
8 KDSC_ECOLI 270 20213 16 (10) 10 (6) 1.94 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1
9 TRYP_PIG 263 25078 7 (7) 4 (4) 0.65 Trypsin OS=Sus scrofa PE=1 SV=1
10 GLDA_ECOLI 261 39087 12 (11) 8 (7) 0.92 Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1
11 YCDX_ECOLI 208 27044 11 (9) 9 (7) 1.25 Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1
12 ATPB_ECOLI 205 50351 12 (10) 12 (10) 0.88 ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2
13 DNAK_ECOLI 203 69130 14 (10) 13 (9) 0.52 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2
14 HEMX_ECOLI 193 42937 7 (6) 6 (5) 0.45 Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1
15 NQOR_ECOLI 186 20832 8 (5) 8 (5) 1.11 NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2
16 AMPA_ECOLI 180 55358 13 (5) 9 (4) 0.33 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1
17 TNAA_ECOLI 156 53139 14 (9) 13 (9) 0.72 Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1
18 GLPQ_ECOLI 153 40874 12 (8) 12 (8) 0.86 Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2
19 PAND_ECOLI 139 13939 5 (4) 3 (2) 0.92 Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1
20 ADHP_ECOLI 134 35870 3 (3) 1 (1) 0.09 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1
21 GLYA_ECOLI 126 45459 16 (8) 14 (7) 0.63 Serine hydroxymethyltransferase OS=Escherichia coli (strain K12) GN=glyA PE=1 SV=1
22 LPP_ECOLI 110 8375 4 (3) 4 (3) 1.87 Major outer membrane lipoprotein Lpp OS=Escherichia coli (strain K12) GN=lpp PE=1 SV=1
23 CISY_ECOLI 106 48383 4 (4) 4 (4) 0.30 Citrate synthase OS=Escherichia coli (strain K12) GN=gltA PE=1 SV=1
24 RPOB_ECOLI 89 150937 2 (1) 2 (1) 0.02 DNA-directed RNA polymerase subunit beta OS=Escherichia coli (strain K12) GN=rpoB PE=1 SV=1
25 ENO_ECOLI 87 45683 9 (5) 8 (4) 0.42 Enolase OS=Escherichia coli (strain K12) GN=eno PE=1 SV=2
26 GMHA_ECOLI 85 20973 5 (2) 5 (2) 0.35 Phosphoheptose isomerase OS=Escherichia coli (strain K12) GN=gmhA PE=1 SV=1
27 EFTU1_ECOLI 83 43427 7 (4) 7 (4) 0.34 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1
28 METK_ECOLI 72 42153 4 (2) 3 (2) 0.16 S-adenosylmethionine synthase OS=Escherichia coli (strain K12) GN=metK PE=1 SV=2
29 PURA_ECOLI 71 47543 5 (4) 5 (4) 0.31 Adenylosuccinate synthetase OS=Escherichia coli (strain K12) GN=purA PE=1 SV=2
30 DEOD_ECOLI 71 26161 1 (1) 1 (1) 0.13 Purine nucleoside phosphorylase DeoD-type OS=Escherichia coli (strain K12) GN=deoD PE=1 SV=2
31 MALY_ECOLI 57 44070 2 (2) 1 (1) 0.07 Protein MalY OS=Escherichia coli (strain K12) GN=malY PE=1 SV=1
32 YAAU_ECOLI 53 0 2 (2) 1 (1) 0.07 description
33 XYLB_ECOLI 51 52927 1 (1) 1 (1) 0.06 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1
34 YKIA_ECOLI 50 0 3 (2) 2 (1) 0.34 description
35 RIR2_ECOLI 49 43775 1 (1) 1 (1) 0.08 Ribonucleoside-diphosphate reductase 1 subunit beta OS=Escherichia coli (strain K12) GN=nrdB PE=1 SV=2
36 YTFJ_ECOLI 44 0 2 (2) 1 (1) 0.16 description
37 FOLB_ECOLI 42 13668 2 (1) 2 (1) 0.25 Dihydroneopterin aldolase OS=Escherichia coli (strain K12) GN=folB PE=1 SV=1
38 G3P1_ECOLI 41 35681 5 (2) 5 (2) 0.19 Glyceraldehyde-3-phosphate dehydrogenase A OS=Escherichia coli (strain K12) GN=gapA PE=1 SV=2
39 CYDA_ECOLI 40 58338 2 (2) 2 (2) 0.12 Cytochrome bd-I ubiquinol oxidase subunit 1 OS=Escherichia coli (strain K12) GN=cydA PE=1 SV=1
40 MNGB_ECOLI 38 100636 5 (3) 1 (1) 0.03 Mannosylglycerate hydrolase OS=Escherichia coli (strain K12) GN=mngB PE=1 SV=2
41 SODF_ECOLI 38 21310 6 (3) 5 (3) 0.55 Superoxide dismutase [Fe] OS=Escherichia coli (strain K12) GN=sodB PE=1 SV=2
42 YJJJ_ECOLI 36 49954 5 (3) 1 (1) 0.07 Putative kinase YjjJ OS=Escherichia coli (strain K12) GN=yjjJ PE=3 SV=1
43 SGCX_ECOLI 35 41102 1 (1) 1 (1) 0.08 Putative aminopeptidase SgcX OS=Escherichia coli (strain K12) GN=sgcX PE=3 SV=3
44 TOLC_ECOLI 34 53708 5 (2) 5 (2) 0.13 Outer membrane protein TolC OS=Escherichia coli (strain K12) GN=tolC PE=1 SV=3
45 MMUM_ECOLI 33 33858 1 (1) 1 (1) 0.10 Homocysteine S-methyltransferase OS=Escherichia coli (strain K12) GN=mmuM PE=1 SV=2
46 RIDA_ECOLI 33 13660 3 (2) 3 (2) 0.56 Enamine/imine deaminase OS=Escherichia coli (strain K12) GN=ridA PE=1 SV=2
47 HEM3_ECOLI 32 34059 1 (1) 1 (1) 0.10 Porphobilinogen deaminase OS=Escherichia coli (strain K12) GN=hemC PE=1 SV=2
48 YOAB_ECOLI 30 12542 6 (1) 4 (1) 0.27 RutC family protein YoaB OS=Escherichia coli (strain K12) GN=yoaB PE=3 SV=1
49 ARAA_ECOLI 28 56437 1 (1) 1 (1) 0.06 L-arabinose isomerase OS=Escherichia coli (strain K12) GN=araA PE=1 SV=3
50 DCUS_ECOLI 27 60627 1 (1) 1 (1) 0.05 Sensor histidine kinase DcuS OS=Escherichia coli (strain K12) GN=dcuS PE=1 SV=1
51 HEMN_ECOLI 27 53209 3 (1) 2 (1) 0.06 Oxygen-independent coproporphyrinogen-III oxidase OS=Escherichia coli (strain K12) GN=hemN PE=1 SV=4
52 BIOD2_ECOLI 26 25079 2 (2) 1 (1) 0.13 ATP-dependent dethiobiotin synthetase BioD 2 OS=Escherichia coli (strain K12) GN=bioD2 PE=3 SV=1
53 NUDK_ECOLI 25 21849 6 (1) 2 (1) 0.15 GDP-mannose pyrophosphatase NudK OS=Escherichia coli (strain K12) GN=nudK PE=1 SV=2
54 EPTA_ECOLI 24 62369 1 (1) 1 (1) 0.05 Phosphoethanolamine transferase EptA OS=Escherichia coli (strain K12) GN=eptA PE=1 SV=2
55 YJCE_ECOLI 23 60484 3 (1) 1 (1) 0.05 Uncharacterized Na(+)/H(+) exchanger YjcE OS=Escherichia coli (strain K12) GN=yjcE PE=3 SV=1
56 YEGI_ECOLI 22 72049 1 (1) 1 (1) 0.05 Uncharacterized protein YegI OS=Escherichia coli (strain K12) GN=yegI PE=4 SV=1
57 FABF_ECOLI 22 43247 3 (2) 3 (2) 0.16 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Escherichia coli (strain K12) GN=fabF PE=1 SV=2
58 PYRD_ECOLI 20 36979 5 (1) 1 (1) 0.09 Dihydroorotate dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=pyrD PE=1 SV=1
59 YQIG_ECOLI 20 0 3 (1) 1 (1) 0.04 description
60 PSTS_ECOLI 20 37001 8 (1) 2 (1) 0.09 Phosphate-binding protein PstS OS=Escherichia coli (strain K12) GN=pstS PE=1 SV=1
61 BFR_ECOLI 19 18483 1 (1) 1 (1) 0.18 Bacterioferritin OS=Escherichia coli (strain K12) GN=bfr PE=1 SV=1
62 KRT83_HUMAN 16 55928 2 (1) 2 (1) 0.06 Keratin, type II cuticular Hb3 OS=Homo sapiens GN=KRT83 PE=1 SV=2
63 POTD_ECOLI 15 38842 2 (1) 1 (1) 0.09 Spermidine/putrescine-binding periplasmic protein OS=Escherichia coli (strain K12) GN=potD PE=1 SV=1

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