MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMN_ECOLI | 27 | Oxygen-independent coproporphyrinogen-III oxidase OS=Escherichia coli (strain K12) GN=hemN PE=1 SV=4 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | BIOD2_ECOLI | 26 | ATP-dependent dethiobiotin synthetase BioD 2 OS=Escherichia coli (strain K12) GN=bioD2 PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUDK_ECOLI | 25 | GDP-mannose pyrophosphatase NudK OS=Escherichia coli (strain K12) GN=nudK PE=1 SV=2 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EPTA_ECOLI | 24 | Phosphoethanolamine transferase EptA OS=Escherichia coli (strain K12) GN=eptA PE=1 SV=2 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJCE_ECOLI | 23 | Uncharacterized Na(+)/H(+) exchanger YjcE OS=Escherichia coli (strain K12) GN=yjcE PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEGI_ECOLI | 22 | Uncharacterized protein YegI OS=Escherichia coli (strain K12) GN=yegI PE=4 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABF_ECOLI | 22 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Escherichia coli (strain K12) GN=fabF PE=1 SV=2 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PYRD_ECOLI | 20 | Dihydroorotate dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=pyrD PE=1 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YQIG_ECOLI | 20 | description |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PSTS_ECOLI | 20 | Phosphate-binding protein PstS OS=Escherichia coli (strain K12) GN=pstS PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 60.1 | PSTS_ECOLI |
20 | 37001 | 8 (1) | 2 (1) | 0.09 | |
| Phosphate-binding protein PstS OS=Escherichia coli (strain K12) GN=pstS PE=1 SV=1 | |||||||
8 peptide matches (3 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2316 |
4 |
549.8099 | 1097.6052 | 1097.6080 | -2.55 | 0 | 8 | 0.3 | 1Score > 15 indicates identity |
U | K.LPSQNIAVVR.R + 2 Deamidated (NQ) |
| 2317 |
1 |
1098.6173 | 1097.6100 | 1097.6080 | 1.80 | 0 | 20 | 0.024 | 1Score > 16 indicates identity |
U | K.LPSQNIAVVR.R + 2 Deamidated (NQ) |
| 2559 |
418.9119 | 1253.7139 | 1253.7092 | 3.76 | 1 | 1 | 1.8 | 1Score > 16 indicates identity |
U | K.LPSQNIAVVRR.A + 2 Deamidated (NQ) |
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