MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1615 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | CATNterm_ECOLX |
324 | 27452 | 14 (13) | 7 (7) | 2.15 | |
| Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 | |||||||
| 2 samesets of CATNterm_ECOLX | |||||||
| CAT_ECOLX |
324 | 25931 | 14 (13) | 7 (7) | 2.35 | ||
| Chloramphenicol acetyltransferase OS=Escherichia coli GN=cat PE=1 SV=1 | |||||||
| CAT_SALTI |
324 | 25931 | 14 (13) | 7 (7) | 2.35 | ||
| Chloramphenicol acetyltransferase OS=Salmonella typhi OX=90370 GN=cat PE=3 SV=1 | |||||||
14 peptide matches (10 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1978 |
1 |
437.7022 | 873.3898 | 873.3868 | 3.44 | 0 | 39 | 0.00014 | 1Score > 13 indicates identity |
U | K.YYTQGDK.V |
| 2368 |
1 |
377.5185 | 1129.5337 | 1129.5338 | -0.15 | 0 | 53 | 1.2e-005 | 1Score > 16 indicates identity |
U | R.LMNAHPEFR.M + Oxidation (M) |
| 2369 |
565.7743 | 1129.5340 | 1129.5338 | 0.18 | 0 | 52 | 1.5e-005 | 1Score > 16 indicates identity |
U | R.LMNAHPEFR.M + Oxidation (M) | |
| 2372 |
377.8471 | 1130.5195 | 1130.5179 | 1.43 | 0 | 38 | 0.00027 | 1Score > 15 indicates identity |
U | R.LMNAHPEFR.M + Deamidated (NQ); Oxidation (M) | |
| 2631 |
1 |
449.9258 | 1346.7556 | 1346.7499 | 4.21 | 0 | 33 | 0.00095 | 1Score > 16 indicates identity |
U | K.FYPAFIHILAR.L |
| 2947 |
1 |
403.9859 | 1611.9145 | 1611.9038 | 6.65 | 1 | 29 | 0.0024 | 1Score > 15 indicates identity |
U | K.HKFYPAFIHILAR.L |
| 3075 |
559.6196 | 1675.8370 | 1675.8318 | 3.07 | 0 | 56 | 1.2e-005 | 1Score > 20 indicates identity |
U | K.ITGYTTVDISQWHR.K | |
| 3139 |
602.3179 | 1803.9319 | 1803.9268 | 2.82 | 1 | 68 | 7.8e-007 | 1Score > 19 indicates identity |
U | K.KITGYTTVDISQWHR.K | |
| 3616 |
475.4532 | 2372.2296 | 2372.1882 | 17.5 | 0 | 44 | 0.00017 | 1Score > 19 indicates identity |
U | K.VLMPLAIQVHHAVCDGFHVGR.M + Deamidated (NQ); Oxidation (M) | |
| 3617 |
594.0655 | 2372.2329 | 2372.1882 | 18.8 | 0 | 23 | 0.021 | 1Score > 19 indicates identity |
U | K.VLMPLAIQVHHAVCDGFHVGR.M + Deamidated (NQ); Oxidation (M) |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
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