MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1615 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | CODA_ECOLI |
463 | 47732 | 24 (19) | 17 (14) | 2.33 | |
| Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 | |||||||
24 peptide matches (23 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1620 |
301.6861 | 601.3576 | 601.3660 | -13.9 | 1 | 9 | 0.15 | 1Score > 21 indicates identity Score > 13 indicates homology |
U | R.RGITR.V | |
| 1763 |
353.6940 | 705.3734 | 705.3731 | 0.50 | 0 | 19 | 0.034 | 1Score > 16 indicates identity |
U | K.AMLEVK.Q + Oxidation (M) | |
| 1821 |
373.7151 | 745.4156 | 745.4123 | 4.54 | 0 | 16 | 0.072 | 1Score > 17 indicates identity |
U | R.AWQTLK.W | |
| 1845 |
385.6917 | 769.3688 | 769.3647 | 5.45 | 0 | 22 | 0.0088 | 1Score > 14 indicates identity |
U | R.FDTYPK.R | |
| 1849 |
389.7276 | 777.4406 | 777.4385 | 2.79 | 0 | 30 | 0.0021 | 1Score > 16 indicates identity |
U | K.TFALAQK.Y | |
| 2215 |
506.2783 | 1010.5420 | 1010.5397 | 2.37 | 0 | 35 | 0.0007 | 1Score > 16 indicates identity |
U | K.ALLTHDDVK.Q | |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube2_A2.rawScore > 16 indicates identity |
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| 2216 |
337.8547 | 1010.5423 | 1010.5397 | 2.59 | 0 | 16 | 0.055 | 1Score > 16 indicates identity |
U | K.ALLTHDDVK.Q | |
| -8.50 | 0 | 2 | 1.3 | 2 | NAAPDIIAAR | ||||||
| 2280 |
354.5148 | 1060.5226 | 1060.5189 | 3.46 | 0 | 13 | 0.26 | 1Score > 19 indicates identity |
U | R.EYGVESLHK.T | |
| 2281 |
531.2687 | 1060.5228 | 1060.5189 | 3.72 | 0 | 35 | 0.0014 | 1Score > 19 indicates identity |
U | R.EYGVESLHK.T | |
| 2507 |
606.8248 | 1211.6350 | 1211.6299 | 4.29 | 1 | 28 | 0.0051 | 1Score > 17 indicates identity |
U | K.TFALAQKYDR.L | |
| 2508 |
404.8857 | 1211.6353 | 1211.6299 | 4.47 | 1 | 38 | 0.00048 | 1Score > 17 indicates identity |
U | K.TFALAQKYDR.L | |
| 2605 |
657.8651 | 1313.7156 | 1313.7051 | 8.01 | 0 | 69 | 5.3e-007 | 1Score > 18 indicates identity |
U | M.SNNALQTIINAR.L | |
| 2611 |
441.5713 | 1321.6921 | 1321.6891 | 2.25 | 0 | 37 | 0.00077 | 1Score > 18 indicates identity |
U | K.WQIANGIQHVR.T + Deamidated (NQ) | |
| 2777 |
731.3661 | 1460.7176 | 1460.7405 | -15.7 | 0 | 1 | 2.9 | 4Score > 20 indicates identity Score > 18 indicates homology |
U | -.MSNNALQTIINAR.L + Oxidation (M) | |
| 2783 |
490.9338 | 1469.7796 | 1469.7726 | 4.76 | 0 | 48 | 5.8e-005 | 1Score > 18 indicates identity |
U | R.THVDVSDATLTALK.A | |
| 2784 |
735.8998 | 1469.7850 | 1469.7726 | 8.48 | 0 | 25 | 0.012 | 1Score > 18 indicates identity |
U | R.THVDVSDATLTALK.A | |
| 3151 |
1 |
453.7283 | 1810.8841 | 1810.8784 | 3.12 | 0 | 39 | 0.00059 | 1Score > 19 indicates identity |
U | R.FVETVAALAHHEGMGAR.V + Oxidation (M) |
| 3391 |
518.7383 | 2070.9241 | 2070.9065 | 8.49 | 0 | 47 | 5.1e-005 | 1Score > 16 indicates identity |
U | R.VTASHTTAMHSYNGAYTSR.L + Deamidated (NQ); Oxidation (M) | |
| 3421 |
699.7089 | 2096.1049 | 2096.0837 | 10.1 | 0 | 73 | 1.4e-007 | 1Score > 17 indicates identity |
U | K.MSGINFVANPLVNIHLQGR.F + Deamidated (NQ); Oxidation (M) | |
| 3920 |
908.1472 | 2721.4198 | 2721.3861 | 12.4 | 1 | 97 | 6.1e-010 | 1Score > 18 indicates identity |
U | K.VIASTQPAQTTVYLEQPEAIDYKR.- + Deamidated (NQ) | |
| 3921 |
681.3643 | 2721.4281 | 2721.3861 | 15.4 | 1 | 25 | 0.011 | 1Score > 18 indicates identity |
U | K.VIASTQPAQTTVYLEQPEAIDYKR.- + Deamidated (NQ) | |
| 4190 |
1101.2596 | 3300.7570 | 3300.7102 | 14.2 | 1 | 39 | 0.00021 | 1Score > 15 indicates identity |
U | R.TLNLQDYGIAAGNSANLIILPAENGFDALRR.Q + Deamidated (NQ) | |
| 4192 |
826.4450 | 3301.7509 | 3301.6942 | 17.2 | 1 | 39 | 0.00016 | 1Score > 14 indicates identity |
U | R.TLNLQDYGIAAGNSANLIILPAENGFDALRR.Q + 2 Deamidated (NQ) | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
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