MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MALY_ECOLI | 57 | Protein MalY OS=Escherichia coli (strain K12) GN=malY PE=1 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YAAU_ECOLI | 53 | description |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLB_ECOLI | 51 | Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YKIA_ECOLI | 50 | description |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RIR2_ECOLI | 49 | Ribonucleoside-diphosphate reductase 1 subunit beta OS=Escherichia coli (strain K12) GN=nrdB PE=1 SV=2 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YTFJ_ECOLI | 44 | description |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FOLB_ECOLI | 42 | Dihydroneopterin aldolase OS=Escherichia coli (strain K12) GN=folB PE=1 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | G3P1_ECOLI | 41 | Glyceraldehyde-3-phosphate dehydrogenase A OS=Escherichia coli (strain K12) GN=gapA PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 38.1 | G3P1_ECOLI |
41 | 35681 | 5 (2) | 5 (2) | 0.19 | |
| Glyceraldehyde-3-phosphate dehydrogenase A OS=Escherichia coli (strain K12) GN=gapA PE=1 SV=2 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1891 |
406.2113 | 810.4080 | 810.4058 | 2.77 | 0 | 8 | 0.36 | 1Score > 16 indicates identity |
U | K.LTGMAFR.V + Oxidation (M) | |
| 1909 |
410.7198 | 819.4250 | 819.4239 | 1.40 | 0 | 23 | 0.02 | 1Score > 18 indicates identity |
U | K.VGINGFGR.I + Deamidated (NQ) | |
| 2330 |
370.2301 | 1107.6685 | 1107.6652 | 2.98 | 0 | 33 | 0.00046 | 1Score > 13 indicates identity |
U | K.VLDLIAHISK.- | |
| 2432 |
581.3274 | 1160.6402 | 1160.6190 | 18.3 | 0 | 12 | 0.25 | 1Score > 19 indicates identity |
U | K.AGIALNDNFVK.L | |
| 2685 |
701.3735 | 1400.7324 | 1400.7259 | 4.65 | 0 | 13 | 0.26 | 1Score > 19 indicates identity |
U | R.GASQNIIPSSTGAAK.A |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| G3P3_ECOLI |
23 | 35799 | 38.1 | ||
| Putative glyceraldehyde-3-phosphate dehydrogenase C OS=Escherichia coli (strain K12) GN=gapC PE=5 SV=2 | |||||
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CYDA_ECOLI | 40 | Cytochrome bd-I ubiquinol oxidase subunit 1 OS=Escherichia coli (strain K12) GN=cydA PE=1 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MNGB_ECOLI | 38 | Mannosylglycerate hydrolase OS=Escherichia coli (strain K12) GN=mngB PE=1 SV=2 |
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