MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 31–40 (out of 63)

Page: Previous 1 2 3 4 5 6 7 Next 

+31

Accession Score Description
1 MALY_ECOLI 57 Protein MalY OS=Escherichia coli (strain K12) GN=malY PE=1 SV=1

+32

Accession Score Description
1 YAAU_ECOLI 53 description

+33

Accession Score Description
1 XYLB_ECOLI 51 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1

+34

Accession Score Description
1 YKIA_ECOLI 50 description

+35

Accession Score Description
1 RIR2_ECOLI 49 Ribonucleoside-diphosphate reductase 1 subunit beta OS=Escherichia coli (strain K12) GN=nrdB PE=1 SV=2

+36

Accession Score Description
1 YTFJ_ECOLI 44 description

-37

Accession Score Description
1 FOLB_ECOLI 42 Dihydroneopterin aldolase OS=Escherichia coli (strain K12) GN=folB PE=1 SV=1
Score Mass Matches Sequences emPAI
37.1 FOLB_ECOLI 42 13668 2 (1) 2 (1) 0.25
Dihydroneopterin aldolase OS=Escherichia coli (strain K12) GN=folB PE=1 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1804   367.7149 733.4152 733.4123 4.07 0 14 0.081 +1Score > 16 indicates identity U R.FALVER.V
2604   656.8792 1311.7438 1311.7398 3.10 0 42 0.00016 +1Score > 16 indicates identity U R.VAEEVAELLLAR.F

+38

Accession Score Description
1 G3P1_ECOLI 41 Glyceraldehyde-3-phosphate dehydrogenase A OS=Escherichia coli (strain K12) GN=gapA PE=1 SV=2

+39

Accession Score Description
1 CYDA_ECOLI 40 Cytochrome bd-I ubiquinol oxidase subunit 1 OS=Escherichia coli (strain K12) GN=cydA PE=1 SV=1

+40

Accession Score Description
1 MNGB_ECOLI 38 Mannosylglycerate hydrolase OS=Escherichia coli (strain K12) GN=mngB PE=1 SV=2
Page: Previous 1 2 3 4 5 6 7 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/