MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA_ECOLI | 126 | Serine hydroxymethyltransferase OS=Escherichia coli (strain K12) GN=glyA PE=1 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LPP_ECOLI | 110 | Major outer membrane lipoprotein Lpp OS=Escherichia coli (strain K12) GN=lpp PE=1 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_ECOLI | 106 | Citrate synthase OS=Escherichia coli (strain K12) GN=gltA PE=1 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_ECOLI | 89 | DNA-directed RNA polymerase subunit beta OS=Escherichia coli (strain K12) GN=rpoB PE=1 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO_ECOLI | 87 | Enolase OS=Escherichia coli (strain K12) GN=eno PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 25.1 | ENO_ECOLI |
87 | 45683 | 9 (5) | 8 (4) | 0.42 | |
| Enolase OS=Escherichia coli (strain K12) GN=eno PE=1 SV=2 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1889 |
403.7323 | 805.4500 | 805.4446 | 6.75 | 0 | 25 | 0.0066 | 1Score > 16 indicates identity |
U | K.YNQLIR.I | |
| 2031 |
464.7975 | 927.5804 | 927.5753 | 5.56 | 0 | 27 | 0.0021 | 1Score > 13 indicates identity |
U | K.GIANSILIK.F | |
| 2034 |
310.5276 | 928.5610 | 928.5593 | 1.80 | 1 | 2 | 1.1 | 2Score > 15 indicates identity |
U | K.ILKEGIEK.G | |
| 2080 |
475.7524 | 949.4902 | 949.4869 | 3.55 | 0 | 16 | 0.094 | 1Score > 18 indicates identity |
U | K.YVLAGEGNK.A | |
| 2465 |
397.2026 | 1188.5860 | 1188.5887 | -2.32 | 0 | 39 | 0.00069 | 1Score > 20 indicates identity |
U | K.DAGYTAVISHR.S | |
| 2466 |
595.3038 | 1188.5930 | 1188.5887 | 3.63 | 0 | 44 | 0.00023 | 1Score > 20 indicates identity |
U | K.DAGYTAVISHR.S | |
| 2571 |
424.5482 | 1270.6228 | 1270.6128 | 7.82 | 0 | 1 | 2.6 | 1Score > 17 indicates identity |
U | R.MGSEVFHHLAK.V + Oxidation (M) | |
| 2807 |
747.4496 | 1492.8846 | 1492.8613 | 15.6 | 0 | 17 | 0.018 | 1Score > 13 indicates identity |
U | K.AVAAVNGPIAQALIGK.D + Deamidated (NQ) | |
| 3443 |
706.6981 | 2117.0725 | 2117.0488 | 11.2 | 0 | 13 | 0.18 | 1Score > 18 indicates identity |
U | R.SGETEDATIADLAVGTAAGQIK.T |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GMHA_ECOLI | 85 | Phosphoheptose isomerase OS=Escherichia coli (strain K12) GN=gmhA PE=1 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 83 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METK_ECOLI | 72 | S-adenosylmethionine synthase OS=Escherichia coli (strain K12) GN=metK PE=1 SV=2 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_ECOLI | 71 | Adenylosuccinate synthetase OS=Escherichia coli (strain K12) GN=purA PE=1 SV=2 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DEOD_ECOLI | 71 | Purine nucleoside phosphorylase DeoD-type OS=Escherichia coli (strain K12) GN=deoD PE=1 SV=2 |
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