MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:38:01 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 63)

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+11

Accession Score Description
1 YCDX_ECOLI 208 Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1

+12

Accession Score Description
1 ATPB_ECOLI 205 ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2

+13

Accession Score Description
1 DNAK_ECOLI 203 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2

+14

Accession Score Description
1 HEMX_ECOLI 193 Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1

+15

Accession Score Description
1 NQOR_ECOLI 186 NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2

-16

Accession Score Description
1 AMPA_ECOLI 180 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1
Score Mass Matches Sequences emPAI
16.1 AMPA_ECOLI 180 55358 13 (5) 9 (4) 0.33
Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1

+13 peptide matches (11 non-duplicate, 2 duplicate)

+17

Accession Score Description
1 TNAA_ECOLI 156 Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1

+18

Accession Score Description
1 GLPQ_ECOLI 153 Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2

+19

Accession Score Description
1 PAND_ECOLI 139 Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1

+20

Accession Score Description
1 ADHP_ECOLI 134 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1
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