MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCDX_ECOLI | 208 | Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_ECOLI | 205 | ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 203 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMX_ECOLI | 193 | Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NQOR_ECOLI | 186 | NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 180 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 16.1 | AMPA_ECOLI |
180 | 55358 | 13 (5) | 9 (4) | 0.33 | |
| Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 | |||||||
13 peptide matches (11 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1620 |
301.6861 | 601.3576 | 601.3548 | 4.82 | 0 | 9 | 0.15 | 1Score > 21 indicates identity Score > 13 indicates homology |
U | K.NVITR.V | |
| 1623 |
302.1779 | 602.3412 | 602.3388 | 4.13 | 0 | 15 | 0.13 | 1Score > 19 indicates identity |
U | K.NVITR.V + Deamidated (NQ) | |
| 1694 |
331.1627 | 660.3108 | 660.3078 | 4.55 | 0 | 8 | 0.53 | 1Score > 18 indicates identity |
R.ELDER.Q | ||
| 1859 |
1 |
396.1995 | 790.3844 | 790.3821 | 3.00 | 0 | 16 | 0.07 | 1Score > 17 indicates identity |
U | R.ELTSGER.A |
| 2378 |
379.2089 | 1134.6049 | 1134.5968 | 7.11 | 1 | 14 | 0.15 | 1Score > 18 indicates identity |
U | K.MVFNVPTRR.E + Oxidation (M) | |
| 2546 |
622.3180 | 1242.6214 | 1242.6132 | 6.63 | 0 | 20 | 0.034 | 1Score > 18 indicates identity |
U | K.ETLYSFDQLK.T | |
| 2566 |
631.8846 | 1261.7546 | 1261.7506 | 3.19 | 0 | 41 | 7.6e-005 | 1Score > 13 indicates identity |
U | R.AIQHGLAIAAGIK.A | |
| 2567 |
421.5934 | 1261.7584 | 1261.7506 | 6.14 | 0 | 59 | 1.4e-006 | 1Score > 13 indicates identity |
U | R.AIQHGLAIAAGIK.A | |
| 3576 |
768.4358 | 2302.2856 | 2302.2420 | 18.9 | 1 | 42 | 6.8e-005 | 1Score > 13 indicates identity |
U | R.LSPIAEQLDKISDGYISALLR.R + Deamidated (NQ) | |
| 3840 |
1 |
536.9044 | 2679.4856 | 2679.4456 | 14.9 | 1 | 7 | 0.2 | 1Score > 13 indicates identity |
U | R.RGELEGKPGQTLLLHHVPNVLSER.I + Deamidated (NQ) |
| 4023 |
933.1262 | 2796.3568 | 2796.3236 | 11.9 | 0 | 79 | 5.5e-008 | 1Score > 19 indicates identity |
U | R.AYRPGDVLTTMSGQTVEVLNTDAEGR.L + Deamidated (NQ); Oxidation (M) |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TNAA_ECOLI | 156 | Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPQ_ECOLI | 153 | Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAND_ECOLI | 139 | Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 134 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
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