MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:38:01 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1615 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 10.1 | GLDA_ECOLI |
261 | 39087 | 12 (11) | 8 (7) | 0.92 | |
| Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 | |||||||
12 peptide matches (9 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1675 |
1 |
324.6829 | 647.3512 | 647.3490 | 3.47 | 0 | 33 | 0.0017 | 1Score > 17 indicates identity |
U | K.TLDTAK.A |
| 1811 |
1 |
371.7281 | 741.4416 | 741.4385 | 4.28 | 0 | 34 | 0.00036 | 1Score > 13 indicates identity |
U | R.IIQSPGK.Y |
| 1825 |
377.7337 | 753.4528 | 753.4497 | 4.17 | 0 | 26 | 0.0028 | 1Score > 13 indicates identity |
U | K.IVAGAPAR.L | |
| 2409 |
1 |
574.8099 | 1147.6052 | 1147.5986 | 5.83 | 0 | 57 | 8.7e-006 | 1Score > 19 indicates identity |
U | K.YIQGADVINR.L |
| 2587 |
430.2481 | 1287.7225 | 1287.7186 | 2.97 | 0 | 39 | 0.00029 | 1Score > 16 indicates identity |
U | R.LGEYLKPLAER.W | |
| 2588 |
644.8687 | 1287.7228 | 1287.7186 | 3.27 | 0 | 23 | 0.0087 | 1Score > 15 indicates identity |
U | R.LGEYLKPLAER.W | |
| 3105 |
584.6430 | 1750.9072 | 1750.9036 | 2.05 | 0 | 28 | 0.0052 | 1Score > 18 indicates identity |
U | K.AMLAAEQHVVTPALER.V + Oxidation (M) | |
| 3116 |
887.9905 | 1773.9664 | 1773.9413 | 14.2 | 0 | 69 | 3.4e-007 | 1Score > 16 indicates identity |
U | R.LLAAGIGDALATWFEAR.A | |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube2_A2.rawScore > 13 indicates identity |
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| 3447 |
708.7272 | 2123.1598 | 2123.1303 | 13.9 | 1 | 12 | 0.074 | 1Score > 13 indicates identity |
U | R.WLVVGDKFVLGFAQSTVEK.S + Deamidated (NQ) | |
| No other peptide matches in query | |||||||||||
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