MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
287 314.1971 313.1898
288 314.1976 313.1903
289 314.1977 313.1904
290 315.0757 314.0684
291 315.1057 314.0984
292 315.1126 314.1053
293 315.1136 314.1063
294 315.1422 314.1349
295 315.1426 314.1353
296 315.1427 314.1354
297 315.1427 314.1354
298 315.1452 314.1379
299 315.1599 314.1526
300 316.0250 315.0177
301 316.0252 315.0179
302 316.0256 315.0183
303 316.2127 315.2054
304 316.2128 315.2055
305 316.2132 315.2059
306 316.2133 315.2060
307 316.2134 315.2061
308 317.0538 316.0465
309 317.0546 316.0473
310 317.1284 316.1211
311 318.0411 317.0338
312 318.1192 317.1119
313 318.1920 317.1847
314 318.1925 317.1852
315 318.1925 317.1852
316 318.1926 317.1853
317 318.1931 317.1858
318 319.0492 318.0419
319 319.1159 318.1086
320 319.1159 318.1086
321 319.1160 318.1087
322 319.1161 318.1088
323 319.1163 318.1090
324 319.1168 318.1095
325 319.1530 318.1457
326 319.1548 318.1475
327 319.1554 318.1481
328 319.1900 318.1827
329 319.2260 318.2187
330 319.9947 318.9874
331 319.9948 318.9875
332 320.1716 319.1643
333 321.1313 320.1240
334 321.1315 320.1242
335 321.1316 320.1243
336 321.1316 320.1243
337 321.1317 320.1244
338 321.1319 320.1246
339 321.1320 320.1247
340 321.1323 320.1250
341 321.1323 320.1250
342 321.1324 320.1251
343 321.1327 320.1254
344 321.1328 320.1255
345 321.1331 320.1258
346 321.1336 320.1263
347 321.1337 320.1264
348 321.1681 320.1608
349 321.1685 320.1612
350 321.1687 320.1614
351 321.1687 320.1614
352 321.1689 320.1616
353 321.1689 320.1616
354 321.1692 320.1619
355 321.1692 320.1619
356 321.1693 320.1620
357 321.1698 320.1625
358 321.1698 320.1625
359 321.1704 320.1631
360 321.1706 320.1633
361 321.1706 320.1633
362 321.2059 320.1986
363 321.2277 320.2204
364 322.1875 321.1802
365 322.1882 321.1809
366 322.1936 321.1863
367 323.0177 322.0104
368 323.0726 322.0653
369 323.1104 322.1031
370 323.1105 322.1032
371 323.1106 322.1033
372 323.1106 322.1033
373 323.1109 322.1036
374 323.1115 322.1042
375 323.1116 322.1043
376 323.1116 322.1043
377 323.1471 322.1398
378 323.1472 322.1399
379 323.1472 322.1399
380 323.1474 322.1401
381 323.1475 322.1402
382 323.1475 322.1402
383 323.1475 322.1402
384 323.1477 322.1404
385 323.1481 322.1408
386 323.1481 322.1408
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