MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4450 836.6465 4178.1961
4451 1045.5634 4178.2245
4452 1045.5646 4178.2293
4453 836.8431 4179.1791
4454 837.2261 4181.0941
4455 837.4234 4182.0806
4456 1051.2886 4201.1253
4457 1053.5198 4210.0501
4458 1053.5232 4210.0637
4460 1064.7916 4255.1373
4461 1065.5070 4257.9989
4462 1065.7430 4258.9429
4463 1070.2438 4276.9461
4464 1077.9979 4307.9625
4467 1082.5048 4325.9901
4471 1086.4969 4341.9585
4474 1088.0020 4347.9789
4475 1088.2484 4348.9645
4477 1088.7603 4351.0121
4478 1090.2518 4356.9781
4479 1090.4955 4357.9529
4480 1090.4977 4357.9617
4481 1091.7460 4362.9549
4482 1091.7562 4362.9957
4483 1091.9979 4363.9625
4484 1092.2479 4364.9625
4485 1092.5067 4365.9977
4486 1092.5122 4366.0197
4487 1092.7524 4366.9805
4488 875.0166 4370.0466
4489 1094.2491 4372.9673
4490 875.6035 4372.9811
4491 730.1997 4375.1545
4492 730.3603 4376.1181
4493 1095.0985 4376.3649
4494 1095.7416 4378.9373
4495 1096.2576 4381.0013
4496 1096.5037 4381.9857
4497 878.4142 4387.0346
4498 879.2804 4391.3656
4499 879.4014 4391.9706
4500 733.2348 4393.3651
4501 1100.2555 4396.9929
4502 1100.5045 4397.9889
4503 880.6177 4398.0521
4504 1103.2554 4408.9925
4506 1103.5740 4410.2669
4507 736.0723 4410.3901
4508 1103.9948 4411.9501
4509 883.4412 4412.1696
4510 1104.5626 4414.2213
4511 1106.0032 4419.9837
4512 1107.9991 4427.9673
4513 739.0190 4428.0703
4514 886.8107 4429.0171
4515 739.1791 4429.0309
4516 739.1834 4429.0567
4518 739.3431 4430.0149
4519 740.2347 4435.3645
4520 1114.7643 4455.0281
4521 1119.5889 4474.3265
4522 895.8735 4474.3311
4523 896.0729 4475.3281
4524 897.6811 4483.3691
4528 1122.1014 4484.3765
4530 899.2749 4491.3381
4531 899.2794 4491.3606
4532 899.2800 4491.3636
4533 749.5683 4491.3661
4534 749.5695 4491.3733
4535 749.5696 4491.3739
4542 643.9217 4500.4010
4545 901.2790 4501.3586
4546 901.2852 4501.3896
4547 901.4938 4502.4326
4548 752.0185 4506.0673
4549 752.0639 4506.3397
4550 902.2775 4506.3511
4551 752.0681 4506.3649
4552 752.1849 4507.0657
4553 752.1855 4507.0693
4554 752.1862 4507.0735
4555 902.4735 4507.3311
4556 902.4775 4507.3511
4557 903.4786 4512.3566
4558 753.5728 4515.3931
4559 753.5751 4515.4069
4560 904.2227 4516.0771
4561 753.6876 4516.0819
4562 904.2286 4516.1066
4563 753.6921 4516.1089
4564 753.6923 4516.1101
4565 904.2828 4516.3776
4567 904.4276 4517.1016
4568 904.4856 4517.3916
4569 646.3496 4517.3963
4570 754.0674 4518.3607
4571 904.6799 4518.3631
4572 904.8834 4519.3806
4573 905.6727 4523.3271
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