MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3610 591.8053 2363.1921
3611 592.0540 2364.1869
3612 474.6452 2368.1896
3613 593.0553 2368.1921
3614 593.3056 2369.1933
3615 474.8460 2369.1936
3618 475.4740 2372.3336
3619 594.0912 2372.3357
3621 795.7090 2384.1052
3624 796.6010 2386.7812
3644 797.4323 2389.2751
3645 599.3027 2393.1817
3647 799.7216 2396.1430
3649 800.0573 2397.1501
3650 800.1085 2397.3037
3651 801.3950 2401.1632
3652 601.5566 2402.1973
3653 601.8049 2403.1905
3654 601.8071 2403.1993
3655 601.8073 2403.2001
3656 601.8076 2403.2013
3657 601.8092 2403.2077
3659 805.1199 2412.3379
3660 805.1212 2412.3418
3661 805.1277 2412.3613
3662 604.8072 2415.1997
3663 806.0742 2415.2008
3664 604.8076 2415.2013
3666 806.4200 2416.2382
3667 605.3156 2417.2333
3668 606.3093 2421.2081
3669 808.4516 2422.3330
3670 808.4547 2422.3423
3671 808.4550 2422.3432
3672 607.2940 2425.1469
3673 486.0370 2425.1486
3674 486.0380 2425.1536
3675 486.2381 2426.1541
3676 813.7827 2438.3263
3677 814.0964 2439.2674
3678 611.0366 2440.1173
3679 489.0309 2440.1181
3680 611.3151 2441.2313
3683 612.0635 2444.2249
3684 612.0657 2444.2337
3686 821.1083 2460.3031
3687 822.1078 2463.3016
3688 822.1084 2463.3034
3690 823.0596 2466.1570
3691 823.1240 2466.3502
3692 826.0681 2475.1825
3693 827.1396 2478.3970
3694 620.8326 2479.3013
3695 827.4413 2479.3021
3697 827.7569 2480.2489
3698 828.0914 2481.2524
3699 828.8266 2483.4580
3700 829.4931 2485.4575
3702 829.7642 2486.2708
3703 830.0970 2487.2692
3704 622.8247 2487.2697
3705 622.8276 2487.2813
3708 831.4150 2491.2232
3709 832.0831 2493.2275
3711 832.4306 2494.2700
3712 625.5882 2498.3237
3713 834.3955 2500.1647
3714 834.4042 2500.1908
3716 836.7426 2507.2060
3726 838.4202 2512.2388
3727 838.4235 2512.2487
3728 838.4259 2512.2559
3729 839.1366 2514.3880
3730 630.0706 2516.2533
3732 842.0880 2523.2422
3733 842.0895 2523.2467
3734 842.0940 2523.2602
3735 843.4194 2527.2364
3736 843.4198 2527.2376
3737 843.7502 2528.2288
3739 634.3098 2533.2101
3740 845.4142 2533.2208
3741 846.0942 2535.2608
3742 635.0872 2536.3197
3744 847.4218 2539.2436
3746 848.0811 2541.2215
3748 848.7495 2543.2267
3751 510.8450 2549.1886
3752 638.3049 2549.1905
3753 510.8455 2549.1911
3755 851.1403 2550.3991
3756 852.1112 2553.3118
3757 852.4211 2554.2415
3758 641.3055 2561.1929
3759 513.2463 2561.1951
3760 855.7242 2564.1508
3761 642.0462 2564.1557
3762 642.0466 2564.1573
3763 514.0393 2565.1601
3764 856.4295 2566.2667
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