MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 3001–3100 (out of 3989)

Page: Previous 1 …26 27 28 29 30 31 32 33 34 35 36 … 40 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3468 721.7152 2162.1238
3469 543.2933 2169.1441
3472 1087.0448 2172.0750
3474 725.3647 2173.0723
3475 1087.5436 2173.0726
3476 725.3676 2173.0810
3477 545.0491 2176.1673
3479 726.6922 2177.0548
3480 727.7021 2180.0845
3481 546.2816 2181.0973
3482 728.0403 2181.0991
3483 546.2827 2181.1017
3484 730.3671 2188.0795
3485 730.7017 2189.0833
3487 734.0266 2199.0580
3488 550.7725 2199.0609
3489 550.7994 2199.1685
3490 735.0217 2202.0433
3491 737.3879 2209.1419
3494 1107.0200 2212.0254
3495 738.3539 2212.0399
3496 554.0806 2212.2933
3497 738.6873 2213.0401
3498 554.3322 2213.2997
3499 741.3578 2221.0516
3500 741.6976 2222.0710
3501 1112.0441 2222.0736
3504 557.5618 2226.2181
3505 744.3547 2230.0423
3506 744.3552 2230.0438
3507 558.8339 2231.3065
3508 558.8347 2231.3097
3509 559.0342 2232.1077
3511 559.5339 2234.1065
3512 746.6974 2237.0704
3517 749.6809 2246.0209
3518 750.0869 2247.2389
3519 1124.6294 2247.2442
3520 750.1079 2247.3019
3521 751.6963 2252.0671
3522 751.7020 2252.0842
3523 564.2854 2253.1125
3524 755.0131 2262.0175
3526 757.0403 2268.0991
3527 568.2795 2269.0889
3528 568.2828 2269.1021
3529 568.5189 2270.0465
3530 757.6951 2270.0635
3531 758.0189 2271.0349
3532 758.3824 2272.1254
3534 758.7023 2273.0851
3535 569.7936 2275.1453
3536 456.2365 2276.1461
3537 570.0460 2276.1549
3538 456.2383 2276.1551
3546 761.0662 2280.1768
3549 762.4420 2284.3042
3550 762.6872 2285.0398
3551 765.0531 2292.1375
3553 765.4176 2293.2310
3554 765.4241 2293.2505
3555 574.5820 2294.2989
3556 574.8345 2295.3089
3557 766.1119 2295.3139
3558 766.4397 2296.2973
3559 766.4412 2296.3018
3560 766.4428 2296.3066
3561 766.7398 2297.1976
3562 766.7404 2297.1994
3563 766.7528 2297.2366
3564 766.7605 2297.2597
3566 767.3526 2299.0360
3567 767.4090 2299.2052
3569 1152.0613 2302.1080
3573 1152.0638 2302.1130
3577 1154.0486 2306.0826
3578 769.7021 2306.0845
3579 1154.0502 2306.0858
3580 769.7032 2306.0878
3581 577.8365 2307.3169
3582 772.1148 2313.3226
3584 580.0218 2316.0581
3585 464.2571 2316.2491
3588 774.0258 2319.0556
3591 776.3553 2326.0441
3592 776.4079 2326.2019
3593 582.7698 2327.0501
3594 582.7704 2327.0525
3595 777.4448 2329.3126
3596 584.0639 2332.2265
3597 585.0912 2336.3357
3600 781.0280 2340.0622
3602 782.3526 2344.0360
3603 587.0172 2344.0397
3604 782.4224 2344.2454
3605 783.0948 2346.2626
3606 784.4565 2350.3477
3607 785.1208 2352.3406
3608 786.0154 2355.0244
3609 787.7519 2360.2339
Page: Previous 1 …26 27 28 29 30 31 32 33 34 35 36 … 40 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/