MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3129 598.9505 1793.8297
3131 897.9540 1793.8934
3134 449.9777 1795.8817
3135 600.0282 1797.0628
3136 601.3126 1800.9160
3137 901.4948 1800.9750
3140 903.9843 1805.9540
3141 602.9966 1805.9680
3142 602.9968 1805.9686
3143 603.3469 1807.0189
3144 603.9891 1808.9455
3147 604.2767 1809.8083
3149 905.9586 1809.9026
3150 906.4077 1810.8008
3154 605.3362 1812.9868
3155 909.4998 1816.9850
3158 607.6576 1819.9510
3161 913.9641 1825.9136
3164 609.9707 1826.8903
3165 914.4583 1826.9020
3170 610.3054 1827.8944
3171 610.3055 1827.8947
3174 610.3321 1827.9745
3175 610.3324 1827.9754
3176 915.0267 1828.0388
3177 915.0281 1828.0416
3178 610.3557 1828.0453
3179 915.4538 1828.8930
3181 917.4846 1832.9546
3189 617.6533 1849.9381
3190 925.9772 1849.9398
3191 463.7379 1850.9225
3193 927.9465 1853.8784
3194 927.9467 1853.8788
3195 619.0029 1853.9869
3197 619.3246 1854.9520
3198 619.3301 1854.9685
3199 621.0319 1860.0739
3201 621.3694 1861.0864
3202 622.0157 1863.0253
3204 622.6411 1864.9015
3205 933.4585 1864.9024
3207 622.6440 1864.9102
3208 374.1886 1865.9066
3209 934.0472 1866.0798
3210 934.0492 1866.0838
3211 934.0505 1866.0864
3212 623.3702 1867.0888
3213 623.3712 1867.0918
3218 938.4514 1874.8882
3219 938.4542 1874.8938
3221 627.0350 1878.0832
3222 627.3699 1879.0879
3223 627.6724 1879.9954
3224 471.2236 1880.8653
3225 941.4407 1880.8668
3226 627.9644 1880.8714
3227 471.2271 1880.8793
3228 629.3345 1884.9817
3230 473.0007 1887.9737
3231 630.3322 1887.9748
3232 630.6655 1888.9747
3233 630.6662 1888.9768
3234 946.8949 1891.7752
3235 947.4953 1892.9760
3236 947.4964 1892.9782
3239 633.9844 1898.9314
3240 634.3498 1900.0276
3241 634.9464 1901.8174
3244 477.2669 1905.0385
3245 636.3334 1905.9784
3246 636.7137 1907.1193
3247 477.9547 1907.7897
3252 638.0399 1911.0979
3253 481.2620 1921.0189
3254 642.3110 1923.9112
3255 643.0356 1926.0850
3257 643.3701 1927.0885
3258 643.3720 1927.0942
3260 643.9869 1928.9389
3262 643.9891 1928.9455
3264 644.6982 1931.0728
3265 644.9866 1931.9380
3271 970.0425 1938.0704
3272 647.0336 1938.0790
3273 970.0499 1938.0852
3274 485.7653 1939.0321
3276 648.3719 1942.0939
3277 648.7016 1943.0830
3278 649.0298 1944.0676
3279 973.5438 1945.0730
3280 649.9476 1946.8210
3281 488.0175 1948.0409
3285 655.0181 1962.0325
3286 982.0245 1962.0344
3287 655.0232 1962.0478
3289 656.0182 1965.0328
3290 394.0146 1965.0366
3291 492.2669 1965.0385
3292 492.2702 1965.0517
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