MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2984 541.3095 1620.9067
2985 407.2044 1624.7885
2989 543.2523 1626.7351
2990 543.2524 1626.7354
2992 543.2925 1626.8557
2993 814.8752 1627.7358
2995 543.9561 1628.8465
2996 815.4370 1628.8594
3000 544.9460 1631.8162
3002 818.3936 1634.7726
3003 545.9315 1634.7727
3005 545.9332 1634.7778
3006 818.4163 1634.8180
3007 546.2241 1635.6505
3008 546.5599 1636.6579
3010 546.5920 1636.7542
3011 819.3856 1636.7566
3012 819.9324 1637.8502
3013 547.5981 1639.7725
3014 821.3855 1640.7564
3015 547.9293 1640.7661
3016 411.1997 1640.7697
3017 411.4493 1641.7681
3018 548.2635 1641.7687
3019 411.6924 1642.7405
3020 548.5876 1642.7410
3021 549.9327 1646.7763
3022 825.9301 1649.8456
3023 826.4252 1650.8358
3024 551.2905 1650.8497
3025 826.4366 1650.8586
3026 552.9174 1655.7304
3027 553.2504 1656.7294
3028 553.2515 1656.7327
3031 553.5836 1657.7290
3032 415.4398 1657.7301
3033 415.4402 1657.7317
3035 554.2866 1659.8380
3036 554.6212 1660.8418
3037 554.6216 1660.8430
3039 556.6252 1666.8538
3040 834.4405 1666.8664
3041 834.9370 1667.8594
3042 834.9373 1667.8600
3043 834.9445 1667.8744
3044 834.9940 1667.9734
3045 556.9990 1667.9752
3046 556.9995 1667.9767
3047 556.9998 1667.9776
3048 835.4926 1668.9706
3049 835.4927 1668.9708
3050 835.4948 1668.9750
3051 835.4954 1668.9762
3052 835.4965 1668.9784
3053 557.3339 1668.9799
3054 557.3348 1668.9826
3055 418.9303 1671.6921
3056 836.8542 1671.6938
3058 836.9405 1671.8664
3059 836.9429 1671.8712
3060 836.9660 1671.9174
3061 558.5721 1672.6945
3063 837.4443 1672.8740
3065 837.4642 1672.9138
3067 558.6462 1672.9168
3068 837.4667 1672.9188
3069 837.4674 1672.9202
3071 558.6479 1672.9219
3072 558.6481 1672.9225
3073 558.6488 1672.9246
3074 559.6079 1675.8019
3077 842.4257 1682.8368
3078 842.4321 1682.8496
3081 570.7191 1709.1355
3082 573.6394 1717.8964
3085 860.9229 1719.8312
3086 575.8743 1724.6011
3087 864.4023 1726.7900
3088 864.4205 1726.8264
3090 576.6808 1727.0206
3093 577.3106 1728.9100
3095 577.3409 1729.0009
3096 866.0082 1730.0018
3098 580.6230 1738.8472
3100 874.9413 1747.8680
3104 875.4391 1748.8636
3106 877.4587 1752.9028
3107 879.4066 1756.7986
3108 879.9144 1757.8142
3110 882.4918 1762.9690
3111 591.6335 1771.8787
3112 886.9473 1771.8800
3113 591.6345 1771.8817
3114 887.4471 1772.8796
3115 887.4495 1772.8844
3117 592.6378 1774.8916
3118 888.4864 1774.9582
3119 592.6603 1774.9591
3127 449.2151 1792.8313
3128 598.6405 1792.8997
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