MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2794 495.5519 1483.6339
2795 495.5536 1483.6390
2797 743.9211 1485.8276
2798 496.5945 1486.7617
2799 496.8972 1487.6698
2800 372.9424 1487.7405
2801 496.9217 1487.7433
2802 496.9450 1487.8132
2803 373.1942 1488.7477
2804 745.8710 1489.7274
2809 500.2715 1497.7927
2810 749.9370 1497.8594
2811 752.3897 1502.7648
2812 752.3900 1502.7654
2813 752.3903 1502.7660
2814 501.9310 1502.7712
2815 376.9641 1503.8273
2816 753.3641 1504.7136
2817 753.3668 1504.7190
2819 502.5862 1504.7368
2821 502.9492 1505.8258
2822 502.9493 1505.8261
2823 753.9234 1505.8322
2826 379.2413 1512.9361
2828 761.3662 1520.7178
2829 507.9460 1520.8162
2830 762.8522 1523.6898
2834 763.9030 1525.7914
2835 763.9032 1525.7918
2836 509.6049 1525.7929
2837 509.6069 1525.7989
2838 764.9280 1527.8414
2839 765.4232 1528.8318
2840 510.6214 1528.8424
2841 765.4290 1528.8434
2842 510.6219 1528.8439
2843 510.6227 1528.8463
2844 765.4305 1528.8464
2851 515.2833 1542.8281
2853 520.2719 1557.7939
2855 781.8810 1561.7474
2856 781.8826 1561.7506
2857 781.8846 1561.7546
2865 522.9183 1565.7331
2866 783.9046 1565.7946
2867 784.8902 1567.7658 1567.7804 -9.25 0     +0Score > 18 indicates identity SIGGVLELVDYMEK + Oxidation (M)
2870 790.8865 1579.7584
2871 790.8865 1579.7584
2872 790.8867 1579.7588
2873 527.5939 1579.7599
2874 790.8894 1579.7642
2876 791.3889 1580.7632
2882 529.2488 1584.7246
2883 529.6109 1585.8109
2888 529.9195 1586.7367
2889 794.3768 1586.7390
2892 530.2502 1587.7288
2894 796.8881 1591.7616
2895 797.3850 1592.7554
2898 797.9072 1593.7998
2900 798.8835 1595.7524
2901 798.8845 1595.7544
2902 798.8870 1595.7594
2903 798.9136 1595.8126
2904 799.3840 1596.7534
2906 800.3770 1598.7394
2908 533.9210 1598.7412
2909 800.4287 1598.8428
2910 800.8748 1599.7350
2911 800.8752 1599.7358
2913 801.8611 1601.7076
2914 401.4348 1601.7101
2915 534.9111 1601.7115
2916 534.9111 1601.7115
2917 534.9268 1601.7586
2939 804.8842 1607.7538
2941 804.8860 1607.7574
2942 805.5029 1608.9912
2943 806.3751 1610.7356
2944 537.9198 1610.7376
2945 806.3776 1610.7406
2948 404.2379 1612.9225
2949 538.9814 1613.9224
2950 539.2505 1614.7297
2951 808.3743 1614.7340
2956 539.8980 1616.6722
2957 809.3436 1616.6726
2958 539.8996 1616.6770
2959 539.9693 1616.8861
2960 809.9114 1617.8082
2966 540.6115 1618.8127
2967 810.4141 1618.8136
2968 540.6126 1618.8160
2969 810.4158 1618.8170
2971 540.6149 1618.8229
2972 810.4191 1618.8236
2973 540.6157 1618.8253
2974 540.6177 1618.8313
2981 541.2369 1620.6889
2983 811.3990 1620.7834
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