MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2551 416.5131 1246.5175
2552 416.5147 1246.5223
2553 417.5157 1249.5253
2557 418.1742 1251.5008
2558 418.9089 1253.7049
2561 419.2213 1254.6421
2568 422.2260 1263.6562
2569 423.2017 1266.5833
2570 318.6622 1270.6197
2574 427.2150 1278.6232
2576 640.8483 1279.6820
2581 428.1932 1281.5578
2582 428.5849 1282.7329
2586 644.3340 1286.6534
2594 433.2389 1296.6949
2595 325.1810 1296.6949
2603 655.8650 1309.7154
2606 658.3371 1314.6596
2608 440.2197 1317.6373
2613 331.6823 1322.7001
2614 663.8851 1325.7556
2615 664.8698 1327.7250
2616 664.8860 1327.7574
2617 664.8914 1327.7682
2619 445.2640 1332.7702
2620 446.9002 1337.6788
2625 671.9091 1341.8036
2626 448.8907 1343.6503
2628 673.8469 1345.6792
2634 675.8513 1349.6880
2635 677.3294 1352.6442
2636 451.8892 1352.6458
2637 452.2362 1353.6868
2638 452.2390 1353.6952
2639 679.8452 1357.6758
2640 679.8469 1357.6792
2646 683.3297 1364.6448
2647 456.1096 1365.3070
2648 685.8530 1369.6914
2650 687.8495 1373.6844
2651 459.2352 1374.6838
2653 459.7530 1376.2372
2655 460.8867 1379.6383
2656 691.3185 1380.6224
2683 700.3296 1398.6446
2687 703.3781 1404.7416
2688 703.3812 1404.7478
2690 471.2425 1410.7057
2691 471.2432 1410.7078
2693 471.9064 1412.6974
2694 471.9067 1412.6983
2696 707.9083 1413.8020
2697 707.9378 1413.8610
2698 472.2954 1413.8644
2699 707.9426 1413.8706
2702 708.8894 1415.7642
2707 709.8689 1417.7232
2708 474.5710 1420.6912
2709 474.5839 1420.7299
2710 711.3737 1420.7328
2712 713.3420 1424.6694
2713 713.3450 1424.6754
2718 357.4523 1425.7801
2719 476.5450 1426.6132
2729 476.8885 1427.6437
2735 358.6881 1430.7233
2742 478.5816 1432.7230
2743 717.3694 1432.7242
2749 718.1229 1434.2312
2750 480.2221 1437.6445
2751 480.8911 1439.6515
2753 481.5558 1441.6456
2754 721.8311 1441.6476
2755 721.8314 1441.6482
2756 481.5579 1441.6519
2757 481.8926 1442.6560
2758 362.1748 1444.6701
2759 482.5651 1444.6735
2761 483.2464 1446.7174
2762 483.5801 1447.7185
2764 725.3669 1448.7192
2765 725.3677 1448.7208
2766 725.3681 1448.7216
2768 728.3429 1454.6712
2769 485.8986 1454.6740
2770 485.8990 1454.6752
2771 364.6761 1454.6753
2772 728.4283 1454.8420
2773 728.4316 1454.8486
2775 728.4338 1454.8530
2776 487.2476 1458.7210
2779 734.9103 1467.8060
2780 734.9104 1467.8062
2781 490.8861 1469.6365
2782 735.8273 1469.6400
2785 491.2448 1470.7126
2788 492.9069 1475.6989
2790 494.5398 1480.5976
2792 371.9151 1483.6313
2793 742.8234 1483.6322
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