MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2334 557.8088 1113.6030
2339 558.2274 1114.4402
2341 558.2801 1114.5456
2342 558.2803 1114.5460
2343 558.7507 1115.4868
2344 558.7527 1115.4908
2345 558.7532 1115.4918
2346 560.2194 1118.4242
2347 373.8162 1118.4268
2349 374.2004 1119.5794
2350 374.2009 1119.5809
2353 374.2175 1119.6307
2356 375.2015 1122.5827
2358 562.7467 1123.4788
2359 375.5300 1123.5682
2360 562.8201 1123.6256
2361 562.8214 1123.6282
2362 562.8231 1123.6316
2363 563.8099 1125.6052
2367 565.7580 1129.5014
2371 377.8400 1130.4982
2373 566.7507 1131.4868
2380 379.5262 1135.5568
2381 379.5275 1135.5607
2382 379.5275 1135.5607
2385 380.5522 1138.6348
2386 570.7133 1139.4120
2387 570.7440 1139.4734
2388 570.7459 1139.4772
2394 381.2067 1140.5983
2398 571.8603 1141.7060
2400 573.7603 1145.5060
2401 382.8524 1145.5354
2402 383.1695 1146.4867
2403 383.1854 1146.5344
2406 383.2417 1146.7033
2407 574.7361 1147.4576
2415 384.2108 1149.6106
2416 384.5144 1150.5214
2417 576.2684 1150.5222
2418 576.2695 1150.5244
2419 384.5156 1150.5250
2420 577.8153 1153.6160
2424 578.7322 1155.4498
2425 578.7429 1155.4712
2426 578.8180 1155.6214
2427 578.8198 1155.6250
2428 386.5374 1156.5904
2429 386.5378 1156.5916
2430 386.5383 1156.5931
2435 387.9111 1160.7115
2436 388.5162 1162.5268
2437 388.5173 1162.5301
2439 583.2510 1164.4874
2440 389.1698 1164.4876
2442 584.7446 1167.4746
2443 390.2120 1167.6142
2448 587.3046 1172.5946
2453 393.5571 1177.6495
2454 589.8324 1177.6502
2456 393.8546 1178.5420
2458 590.7720 1179.5294
2459 394.1912 1179.5518
2460 394.8806 1181.6200
2461 591.8422 1181.6698
2464 397.1851 1188.5335
2467 397.4985 1189.4737
2468 597.2340 1192.4534
2469 597.2371 1192.4596
2471 597.8050 1193.5954
2479 597.8117 1193.6088
2484 399.1775 1194.5107
2485 598.3835 1194.7524
2487 400.2130 1197.6172
2489 400.2149 1197.6229
2490 600.3438 1198.6730
2491 601.7951 1201.5756
2493 602.3111 1202.6076
2498 603.8306 1205.6466
2504 404.5468 1210.6186
2506 404.5476 1210.6210
2509 608.7997 1215.5848
2511 406.8618 1217.5636
2512 610.3456 1218.6766
2514 610.8200 1219.6254
2515 610.8210 1219.6274
2516 610.8369 1219.6592
2518 612.2719 1222.5292
2521 409.5754 1225.7044
2522 409.5754 1225.7044
2524 409.5762 1225.7068
2533 411.5243 1231.5511
2534 308.8954 1231.5525
2535 309.1460 1232.5549
2537 618.2393 1234.4640
2539 618.3229 1234.6312
2541 412.5645 1234.6717
2543 618.8189 1235.6232
2544 619.9135 1237.8124
2545 619.9147 1237.8148
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