MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2130 486.7927 971.5708
2131 487.2044 972.3942
2132 325.1390 972.3952
2133 487.2051 972.3956
2134 325.1392 972.3958
2135 973.4996 972.4923
2136 487.7648 973.5150
2139 488.7324 975.4502
2140 488.7325 975.4504
2141 490.7297 979.4448
2142 490.7298 979.4450
2143 981.4077 980.4004
2144 491.2075 980.4004
2147 327.8365 980.4877
2148 327.8370 980.4892
2149 327.8375 980.4907
2150 491.2856 980.5566
2151 491.2861 980.5576
2159 493.1917 984.3688
2168 329.8015 986.3827
2169 329.8020 986.3842
2170 494.1994 986.3842
2171 494.1998 986.3850
2173 494.2601 986.5056
2174 494.8147 987.6148
2175 495.2469 988.4792
2176 495.2562 988.4978
2177 330.5364 988.5874
2179 330.8642 989.5708
2182 331.8079 992.4019
2183 497.2304 992.4462
2188 498.7333 995.4520
2189 332.8247 995.4523
2190 332.8248 995.4526
2191 498.7338 995.4530
2192 332.8401 995.4985
2197 501.6816 1001.3486
2198 334.7902 1001.3488
2199 501.6823 1001.3500
2204 335.1358 1002.3856
2205 335.7933 1004.3581
2206 503.1866 1004.3586
2208 503.7902 1005.5658
2210 504.7845 1007.5544
2211 1009.5295 1008.5222
2212 337.1870 1008.5392
2213 337.1875 1008.5407
2214 505.7791 1009.5436
2220 508.7814 1015.5482
2222 509.7882 1017.5618
2223 509.7888 1017.5630
2224 509.7896 1017.5646
2225 509.7899 1017.5652
2226 509.7903 1017.5660
2227 511.7241 1021.4336
2228 511.7241 1021.4336
2229 342.1804 1023.5194
2230 512.7675 1023.5204
2231 512.7679 1023.5212
2233 513.2461 1024.4776
2234 513.3038 1024.5930
2235 342.8634 1025.5684
2245 519.1146 1036.2146
2246 519.3168 1036.6190
2249 1038.0243 1037.0170
2250 1038.0540 1037.0467
2251 347.1685 1038.4837
2252 520.2504 1038.4862
2255 347.8552 1040.5438
2256 348.1766 1041.5080
2259 348.5112 1042.5118
2261 522.3056 1042.5966
2262 522.7620 1043.5094
2263 522.7620 1043.5094
2272 1051.5392 1050.5319
2273 526.5140 1051.0134
2274 528.2759 1054.5372
2275 529.2333 1056.4520
2276 530.2229 1058.4312
2277 530.2233 1058.4320
2278 530.2452 1058.4758
2284 354.8414 1061.5024
2286 355.8470 1064.5192
2294 358.8330 1073.4772
2295 358.8336 1073.4790
2297 538.2206 1074.4266
2298 540.8077 1079.6008
2301 542.3208 1082.6270
2302 361.8834 1082.6284
2308 1093.5461 1092.5388
2312 547.8282 1093.6418
2313 547.8285 1093.6424
2314 366.1994 1095.5764
2315 366.1998 1095.5776
2323 550.3301 1098.6456
2325 368.5182 1102.5328
2326 553.7310 1105.4474
2327 369.4906 1105.4500
2329 554.2332 1106.4518
2333 370.8363 1109.4871
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