MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1864 399.1771 796.3396
1866 799.0677 798.0604
1867 400.2289 798.4432
1890 404.7581 807.5016
1892 407.6966 813.3786
1894 408.6866 815.3586
1895 408.7047 815.3948
1897 816.4832 815.4759
1903 409.6937 817.3728
1905 409.6947 817.3748
1907 409.7536 817.4926
1908 410.7134 819.4122
1911 411.2713 820.5280
1913 412.6901 823.3656
1917 412.7546 823.4946
1918 825.4642 824.4569
1919 825.4706 824.4633
1920 413.6775 825.3404
1921 827.0879 826.0806
1922 827.3916 826.3843
1923 415.6941 829.3736
1924 415.6956 829.3766
1932 834.4432 833.4359
1934 836.7509 835.7436
1935 420.6714 839.3282
1937 842.5128 841.5055
1942 422.7096 843.4046
1943 423.2008 844.3870
1945 424.7109 847.4072
1946 848.4146 847.4073
1947 425.1813 848.3480
1948 849.4305 848.4232
1949 427.7226 853.4306
1950 855.2982 854.2909
1951 428.1537 854.2928
1952 428.1544 854.2942
1953 428.7029 855.3912
1956 857.0936 856.0863
1957 430.2079 858.4012
1958 430.2086 858.4026
1960 431.2098 860.4050
1961 431.2628 860.5110
1962 864.4260 863.4187
1963 435.2040 868.3934
1964 435.2359 868.4572
1965 435.2364 868.4582
1967 435.7749 869.5352
1968 435.7761 869.5376
1972 437.2120 872.4094
1976 874.3940 873.3867
1983 879.4026 878.3953
1984 883.1461 882.1388
1985 443.2257 884.4368
1989 891.4247 890.4174
1993 449.7090 897.4034
1994 449.7372 897.4598
1998 450.2237 898.4328
2000 451.6858 901.3570
2001 452.2308 902.4470
2002 302.8697 905.5873
2003 454.2141 906.4136
2008 303.8841 908.6305
2009 303.8856 908.6350
2010 910.4810 909.4737
2011 912.4643 911.4570
2023 308.1964 921.5674
2025 463.2713 924.5280
2027 464.2681 926.5216
2030 310.1919 927.5539
2032 465.2480 928.4814
2035 465.7282 929.4418
2037 466.2207 930.4268
2038 466.2209 930.4272
2039 466.2299 930.4452
2040 311.1796 930.5170
2048 468.7334 935.4522
2049 468.7350 935.4554
2070 474.7463 947.4780
2071 474.7555 947.4964
2086 478.1152 954.2158
2089 480.2740 958.5334
2091 480.2758 958.5370
2094 960.5001 959.4928
2098 321.8665 962.5777
2099 321.8665 962.5777
2106 965.4369 964.4296
2107 483.2229 964.4312
2108 483.2234 964.4322
2109 483.2575 964.5004
2111 322.5107 964.5103
2112 484.2069 966.3992
2113 484.2082 966.4018
2117 323.1735 966.4987
2118 323.1736 966.4990
2120 485.2839 968.5532
2121 485.2857 968.5568
2123 485.2873 968.5600
2124 324.1416 969.4030
2126 485.7425 969.4704
2127 485.7443 969.4740
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