MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1631 304.8356 607.6566
1632 304.8357 607.6568
1633 304.8361 607.6576
1634 306.1553 610.2960
1636 306.7015 611.3884
1638 308.1959 614.3772
1640 616.3781 615.3708
1645 311.7004 621.3862
1655 625.7707 624.7634
1659 631.3439 630.3366
1662 635.3525 634.3452
1663 635.3539 634.3466
1665 319.1514 636.2882
1666 320.1349 638.2552
1667 320.7057 639.3968
1668 320.7068 639.3990
1669 321.3226 640.6306
1670 323.1465 644.2784
1673 648.3582 647.3509
1676 325.1646 648.3146
1677 325.3493 648.6840
1680 325.6801 649.3456
1681 325.7164 649.4182
1682 325.7166 649.4186
1685 654.3986 653.3913
1686 328.1561 654.2976
1688 329.1715 656.3284
1691 657.4049 656.3976
1699 666.2850 665.2777
1700 334.2161 666.4176
1701 334.2163 666.4180
1703 336.1783 670.3420
1705 336.6698 671.3250
1706 672.3335 671.3262
1707 336.6708 671.3270
1713 672.4056 671.3983
1714 337.1250 672.2354
1715 337.2034 672.3922
1718 338.0094 674.0042
1722 338.1941 674.3736
1723 339.1643 676.3140
1724 341.1583 680.3020
1725 341.2192 680.4238
1726 341.2195 680.4244
1727 341.2205 680.4264
1729 341.6617 681.3088
1731 342.7859 683.5572
1733 685.3528 684.3455
1734 343.1803 684.3460
1735 344.2098 686.4050
1751 346.2126 690.4106
1756 349.6876 697.3606
1757 350.1790 698.3434
1759 350.7284 699.4422
1761 351.6758 701.3370
1762 706.3661 705.3588
1767 709.3719 708.3646
1768 356.1680 710.3214
1769 711.3400 710.3327
1770 712.2565 711.2492
1771 357.1525 712.2904
1772 357.1626 712.3106
1775 358.2216 714.4286
1785 362.2001 722.3856
1788 363.1856 724.3566
1789 363.1857 724.3568
1790 726.4061 725.3988
1791 726.4072 725.3999
1792 363.7168 725.4190
1794 364.1567 726.2988
1795 364.1573 726.3000
1796 730.4114 729.4041
1797 365.7094 729.4042
1805 735.3671 734.3598
1806 370.6664 739.3182
1807 371.6393 741.2640
1808 371.6395 741.2644
1809 371.6859 741.3572
1812 743.4437 742.4364
1815 744.4370 743.4297
1816 744.4397 743.4324
1818 373.1436 744.2726
1819 373.1437 744.2728
1822 374.2916 746.5686
1824 377.2144 752.4142
1826 378.6654 755.3162
1827 379.1377 756.2608
1838 763.3788 762.3715
1840 382.7010 763.3874
1841 383.1864 764.3582
1842 765.4335 764.4262
1844 384.7013 767.3880
1846 386.7194 771.4242
1847 387.6919 773.3692
1853 782.4213 781.4140
1854 392.6915 783.3684
1855 392.6923 783.3700
1856 393.2195 784.4244
1862 795.3282 794.3209
1863 795.3287 794.3214
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