MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1489 472.3259 471.3186
1490 478.2770 477.2697
1491 479.7126 478.7053
1492 484.1426 483.1353
1493 485.8132 484.8059
1494 486.2690 485.2617
1495 486.2690 485.2617
1496 487.3000 486.2927
1497 487.8047 486.7974
1499 488.2848 487.2775
1504 490.2622 489.2549
1505 490.2632 489.2559
1507 490.2638 489.2565
1508 490.8202 489.8129
1509 490.8211 489.8138
1510 491.2590 490.2517
1511 493.0206 492.0133
1512 493.0208 492.0135
1513 493.0210 492.0137
1514 493.0213 492.0140
1515 493.0215 492.0142
1516 493.0218 492.0145
1517 494.8133 493.8060
1518 494.8142 493.8069
1519 494.8145 493.8072
1520 494.8152 493.8079
1521 496.7134 495.7061
1522 496.7138 495.7065
1523 497.8936 496.8863
1525 503.2958 502.2885
1527 504.2782 503.2709
1529 504.2793 503.2720
1530 504.2799 503.2726
1531 509.2251 508.2178
1532 510.2571 509.2498
1533 510.2576 509.2503
1534 510.2677 509.2604
1535 510.2683 509.2610
1536 511.3253 510.3180
1537 511.3257 510.3184
1538 512.1356 511.1283
1539 512.1378 511.1305
1540 513.3047 512.2974
1541 513.3049 512.2976
1542 513.3179 512.3106
1543 514.2626 513.2553
1544 514.2632 513.2559
1545 514.2636 513.2563
1546 514.2640 513.2567
1548 515.3325 514.3252
1549 518.2593 517.2520
1550 520.7393 519.7320
1551 521.2509 520.2436
1552 521.2529 520.2456
1553 524.0491 523.0418
1554 529.3464 528.3391
1555 531.2911 530.2838
1559 534.6949 533.6876
1560 536.2727 535.2654
1561 538.2997 537.2924
1562 538.3000 537.2927
1563 538.3005 537.2932
1565 542.1461 541.1388
1566 542.2514 541.2441
1567 542.3069 541.2996
1572 550.3011 549.2938
1573 554.3405 553.3332
1574 557.2578 556.2505
1575 559.3194 558.3121
1578 560.3051 559.2978
1582 560.3176 559.3103
1585 568.7194 567.7121
1586 568.7197 567.7124
1587 568.7203 567.7130
1588 570.3248 569.3175
1589 570.7139 569.7066
1590 570.7141 569.7068
1591 570.7144 569.7071
1592 570.7145 569.7072
1593 570.7145 569.7072
1594 570.7145 569.7072
1595 570.7147 569.7074
1596 570.7156 569.7083
1597 570.7177 569.7104
1604 576.6486 575.6413
1605 576.6808 575.6735
1606 581.3449 580.3376
1607 585.3008 584.2935
1608 585.3013 584.2940
1609 594.6461 593.6388
1610 596.6402 595.6329
1611 596.6408 595.6335
1612 597.3605 596.3532
1615 600.4202 599.4129
1621 602.8391 601.8318
1625 303.8384 605.6622
1626 303.8858 605.7570
1627 304.1459 606.2772
1629 304.8349 607.6552
1630 304.8354 607.6562
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