MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1388 431.2627 430.2554
1390 432.8503 431.8430
1391 432.9530 431.9457
1392 434.2256 433.2183
1393 435.0742 434.0669
1394 437.0899 436.0826
1395 437.2156 436.2083
1396 437.2169 436.2096
1397 437.2172 436.2099
1398 437.2183 436.2110
1399 437.2195 436.2122
1400 439.1845 438.1772
1401 439.1959 438.1886
1402 439.2137 438.2064
1403 439.2141 438.2068
1404 439.2143 438.2070
1405 439.2151 438.2078
1406 439.2155 438.2082
1407 439.2157 438.2084
1408 439.2158 438.2085
1409 439.2158 438.2085
1410 439.2160 438.2087
1411 439.2161 438.2088
1412 439.2165 438.2092
1413 439.2169 438.2096
1414 439.2198 438.2125
1415 439.2305 438.2232
1416 439.2322 438.2249
1417 439.2327 438.2254
1418 439.2332 438.2259
1419 439.2336 438.2263
1420 439.2352 438.2279
1421 441.2308 440.2235
1422 441.2314 440.2241
1423 441.2318 440.2245
1424 441.2336 440.2263
1425 442.2664 441.2591
1426 443.2467 442.2394
1427 443.2468 442.2395
1428 443.2470 442.2397
1429 443.2475 442.2402
1430 443.2477 442.2404
1431 444.1094 443.1021
1432 445.2685 444.2612
1433 446.2729 445.2656
1434 446.2741 445.2668
1435 446.2757 445.2684
1436 446.2759 445.2686
1437 447.2315 446.2242
1438 447.2325 446.2252
1439 447.7931 446.7858
1440 448.3120 447.3047
1441 448.3124 447.3051
1442 448.3127 447.3054
1443 448.3134 447.3061
1444 449.1519 448.1446
1445 451.1678 450.1605
1446 453.2130 452.2057
1447 453.7875 452.7802
1448 453.7883 452.7810
1449 455.2639 454.2566
1450 456.2940 455.2867
1451 456.2946 455.2873
1452 456.2946 455.2873
1453 457.2256 456.2183
1454 457.2271 456.2198
1455 457.2277 456.2204
1456 457.2887 456.2814
1457 458.2728 457.2655
1458 458.2737 457.2664
1459 458.2743 457.2670
1460 458.2772 457.2699
1461 459.7524 458.7451
1462 459.7534 458.7461
1463 459.7536 458.7463
1464 461.2570 460.2497
1465 464.0271 463.0198
1466 465.8077 464.8004
1467 465.8086 464.8013
1468 466.0424 465.0351
1469 466.0425 465.0352
1470 466.3229 465.3156
1471 467.8020 466.7947
1472 467.8027 466.7954
1473 467.8028 466.7955
1474 467.8028 466.7955
1475 467.8029 466.7956
1476 467.8031 466.7958
1477 467.8036 466.7963
1478 467.8040 466.7967
1479 467.8042 466.7969
1480 470.1252 469.1179
1481 471.7718 470.7645
1482 471.7984 470.7911
1483 471.7990 470.7917
1484 472.2531 471.2458
1485 472.2900 471.2827
1486 472.3246 471.3173
1487 472.3251 471.3178
1488 472.3252 471.3179
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