MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1287 407.2255 406.2182
1288 407.2256 406.2183
1289 407.2260 406.2187
1290 407.2260 406.2187
1291 407.2263 406.2190
1292 407.2265 406.2192
1293 409.1839 408.1766
1294 409.1840 408.1767
1295 409.1845 408.1772
1296 409.1850 408.1777
1297 409.1851 408.1778
1298 409.1851 408.1778
1299 409.1853 408.1780
1300 409.1864 408.1791
1301 409.1879 408.1806
1302 409.1881 408.1808
1303 409.2402 408.2329
1304 409.2412 408.2339
1305 409.2412 408.2339
1306 409.2414 408.2341
1307 409.2415 408.2342
1308 409.2415 408.2342
1309 409.2426 408.2353
1310 410.2447 409.2374
1311 411.1999 410.1926
1312 411.2001 410.1928
1313 411.2003 410.1930
1314 411.2003 410.1930
1315 411.2010 410.1937
1316 411.2025 410.1952
1317 411.2571 410.2498
1318 412.9065 411.8992
1319 415.0923 414.0850
1320 415.1308 414.1235
1321 415.1555 414.1482
1322 416.2627 415.2554
1323 416.2883 415.2810
1324 416.2884 415.2811
1325 416.3746 415.3673
1326 416.3749 415.3676
1327 417.2467 416.2394
1329 417.2729 416.2656
1330 418.2464 417.2391
1331 419.1901 418.1828
1332 421.2031 420.1958
1333 421.2033 420.1960
1334 421.2034 420.1961
1335 421.2041 420.1968
1336 421.2052 420.1979
1337 421.2059 420.1986
1338 423.2000 422.1927
1339 423.2009 422.1936
1340 423.2018 422.1945
1341 423.2032 422.1959
1342 423.2035 422.1962
1343 423.2178 422.2105
1344 423.2180 422.2107
1345 423.2183 422.2110
1346 423.2188 422.2115
1347 423.2196 422.2123
1348 423.2201 422.2128
1349 423.2203 422.2130
1350 423.2206 422.2133
1351 423.2207 422.2134
1352 423.2207 422.2134
1353 423.2208 422.2135
1354 423.2209 422.2136
1355 423.2219 422.2146
1356 423.2220 422.2147
1357 423.2230 422.2157
1358 425.1782 424.1709
1359 425.1805 424.1732
1360 425.1806 424.1733
1361 425.1811 424.1738
1362 425.2148 424.2075
1363 425.2158 424.2085
1364 425.2169 424.2096
1365 425.2356 424.2283
1366 425.2362 424.2289
1367 425.2364 424.2291
1368 425.2365 424.2292
1369 425.2367 424.2294
1370 425.2371 424.2298
1371 425.7925 424.7852
1372 427.2521 426.2448
1373 427.2558 426.2485
1374 427.2569 426.2496
1375 428.2545 427.2472
1376 429.0896 428.0823
1377 429.1985 428.1912
1378 429.7366 428.7293
1379 430.0935 429.0862
1380 430.0953 429.0880
1381 430.2087 429.2014
1382 430.3011 429.2938
1383 430.3014 429.2941
1384 430.3016 429.2943
1385 430.3018 429.2945
1386 430.3021 429.2948
1387 430.3023 429.2950
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