MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1187 390.2096 389.2023
1188 390.2500 389.2427
1189 390.2504 389.2431
1190 391.2857 390.2784
1191 392.0051 390.9978
1192 393.0889 392.0816
1193 393.1531 392.1458
1194 393.1531 392.1458
1195 393.1537 392.1464
1196 393.1540 392.1467
1197 393.1544 392.1471
1198 393.1897 392.1824
1199 393.1898 392.1825
1200 393.1910 392.1837
1201 393.1920 392.1847
1202 393.1924 392.1851
1203 393.1930 392.1857
1204 393.2111 392.2038
1205 393.2457 392.2384
1206 393.2983 392.2910
1207 393.2988 392.2915
1208 393.2988 392.2915
1209 393.2991 392.2918
1210 393.2997 392.2924
1211 393.2998 392.2925
1212 393.3000 392.2927
1213 393.3005 392.2932
1214 393.3005 392.2932
1215 393.3006 392.2933
1216 393.3006 392.2933
1217 393.3009 392.2936
1218 393.3014 392.2941
1219 393.3015 392.2942
1220 395.0759 394.0686
1221 395.0766 394.0693
1222 395.1688 394.1615
1223 395.1688 394.1615
1224 395.1690 394.1617
1225 395.1692 394.1619
1226 395.1694 394.1621
1227 395.1695 394.1622
1228 395.1697 394.1624
1229 395.1698 394.1625
1230 395.1700 394.1627
1231 395.1702 394.1629
1232 395.1721 394.1648
1233 395.2043 394.1970
1234 395.2050 394.1977
1235 395.2051 394.1978
1236 395.2053 394.1980
1237 395.2062 394.1989
1238 395.2077 394.2004
1239 395.2080 394.2007
1240 397.1841 396.1768
1241 397.1845 396.1772
1242 397.1847 396.1774
1243 397.1853 396.1780
1244 397.1856 396.1783
1245 397.1857 396.1784
1246 397.1861 396.1788
1247 397.1861 396.1788
1248 397.1873 396.1800
1249 397.1877 396.1804
1250 397.8822 396.8749
1251 398.2160 397.2087
1252 398.2393 397.2320
1253 398.2403 397.2330
1254 398.2417 397.2344
1255 398.2422 397.2349
1256 399.0984 398.0911
1257 399.2210 398.2137
1258 399.2365 398.2292
1259 399.2365 398.2292
1260 399.7469 398.7396
1261 400.0822 399.0749
1262 400.2308 399.2235
1263 401.1143 400.1070
1264 401.2871 400.2798
1265 401.2876 400.2803
1266 401.2879 400.2806
1267 401.2885 400.2812
1268 401.7068 400.6995
1269 402.0619 401.0546
1270 402.2465 401.2392
1271 402.2468 401.2395
1272 402.2474 401.2401
1273 402.2475 401.2402
1274 402.2480 401.2407
1275 403.2310 402.2237
1276 403.8110 402.8037
1277 404.2157 403.2084
1278 404.2160 403.2087
1279 405.0696 404.0623
1280 405.2276 404.2203
1281 407.1685 406.1612
1282 407.1702 406.1629
1283 407.1703 406.1630
1284 407.1710 406.1637
1285 407.2250 406.2177
1286 407.2254 406.2181
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