MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1087 375.1493 374.1420
1088 376.2343 375.2270
1089 376.2344 375.2271
1090 376.2345 375.2272
1091 376.2349 375.2276
1092 377.1580 376.1507
1093 377.1583 376.1510
1094 377.1584 376.1511
1095 377.1591 376.1518
1096 377.1597 376.1524
1097 377.1599 376.1526
1098 377.1613 376.1540
1099 377.8641 376.8568
1100 377.9901 376.9828
1101 378.0236 377.0163
1102 379.1387 378.1314
1103 379.1391 378.1318
1104 379.1723 378.1650
1105 379.1734 378.1661
1106 379.1735 378.1662
1107 379.1740 378.1667
1108 379.1742 378.1669
1109 379.1743 378.1670
1110 379.1744 378.1671
1111 379.1750 378.1677
1112 379.1750 378.1677
1113 379.1757 378.1684
1114 379.1766 378.1693
1115 379.1770 378.1697
1116 379.8591 378.8518
1117 380.2303 379.2230
1118 380.9658 379.9585
1119 381.1531 380.1458
1120 381.1534 380.1461
1121 381.1536 380.1463
1122 381.1539 380.1466
1123 381.1541 380.1468
1124 381.1541 380.1468
1125 381.1545 380.1472
1126 381.1891 380.1818
1127 381.1893 380.1820
1128 381.1894 380.1821
1129 381.1895 380.1822
1130 381.1897 380.1824
1131 381.1898 380.1825
1132 381.1901 380.1828
1133 381.1904 380.1831
1134 381.1904 380.1831
1135 381.1905 380.1832
1136 381.1908 380.1835
1137 381.1913 380.1840
1138 381.1913 380.1840
1139 381.1924 380.1851
1140 381.1924 380.1851
1141 381.1927 380.1854
1142 381.2098 380.2025
1143 381.2107 380.2034
1144 381.2107 380.2034
1145 381.2123 380.2050
1146 383.1681 382.1608
1147 383.1689 382.1616
1148 383.1689 382.1616
1149 383.1693 382.1620
1150 383.1697 382.1624
1151 383.1699 382.1626
1152 383.1701 382.1628
1153 383.1703 382.1630
1154 383.2052 382.1979
1155 383.2054 382.1981
1156 383.2062 382.1989
1157 383.2065 382.1992
1158 383.2072 382.1999
1159 383.2073 382.2000
1160 383.2077 382.2004
1161 383.2083 382.2010
1162 383.2260 382.2187
1163 385.0382 384.0309
1164 385.1183 384.1110
1165 385.1202 384.1129
1166 385.2201 384.2128
1167 385.2208 384.2135
1168 385.2208 384.2135
1169 385.2447 384.2374
1170 386.0673 385.0600
1171 386.0673 385.0600
1172 386.0676 385.0603
1173 386.0686 385.0613
1174 387.0984 386.0911
1175 387.1367 386.1294
1176 388.0475 387.0402
1177 388.2305 387.2232
1178 388.2317 387.2244
1179 388.2707 387.2634
1180 388.2719 387.2646
1181 388.2734 387.2661
1182 389.2158 388.2085
1183 389.2523 388.2450
1184 389.8175 388.8102
1185 390.0629 389.0556
1186 390.0636 389.0563
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