MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
987 367.1928 366.1855
988 367.1929 366.1856
989 367.1936 366.1863
990 367.2116 366.2043
991 367.2119 366.2046
992 367.2120 366.2047
993 367.2120 366.2047
994 367.2121 366.2048
995 367.2126 366.2053
996 367.2321 366.2248
997 367.2324 366.2251
998 367.2326 366.2253
999 367.2326 366.2253
1000 367.2327 366.2254
1001 367.2328 366.2255
1002 367.2328 366.2255
1003 367.2466 366.2393
1004 368.8265 367.8192
1005 368.8415 367.8342
1006 369.0876 368.0803
1007 369.0878 368.0805
1008 369.1515 368.1442
1009 369.1530 368.1457
1010 369.1531 368.1458
1011 369.1535 368.1462
1012 369.1536 368.1463
1013 369.1537 368.1464
1014 369.1547 368.1474
1015 369.1548 368.1475
1016 369.2092 368.2019
1017 369.2272 368.2199
1018 369.2272 368.2199
1019 369.2276 368.2203
1020 369.2276 368.2203
1021 369.2277 368.2204
1022 369.2278 368.2205
1023 369.2282 368.2209
1024 369.2283 368.2210
1025 369.2480 368.2407
1026 369.2486 368.2413
1027 369.2486 368.2413
1028 369.2486 368.2413
1029 370.0279 369.0206
1030 370.0284 369.0211
1031 370.0347 369.0274
1032 370.0723 369.0650
1033 370.8373 369.8300
1034 371.0140 370.0067
1035 371.1035 370.0962
1036 371.1041 370.0968
1037 371.2281 370.2208
1038 371.3157 370.3084
1039 371.3160 370.3087
1040 371.3161 370.3088
1041 371.3163 370.3090
1042 371.3164 370.3091
1043 371.3165 370.3092
1044 371.3166 370.3093
1045 371.3167 370.3094
1046 371.3167 370.3094
1047 371.3168 370.3095
1048 371.3169 370.3096
1049 371.3170 370.3097
1050 371.3170 370.3097
1051 371.3172 370.3099
1052 371.3172 370.3099
1053 371.3173 370.3100
1054 371.3173 370.3100
1055 371.3173 370.3100
1056 371.3173 370.3100
1057 371.3173 370.3100
1058 371.3177 370.3104
1059 371.3177 370.3104
1060 371.3179 370.3106
1061 371.3181 370.3108
1062 371.3181 370.3108
1063 371.3182 370.3109
1064 371.3183 370.3110
1065 371.3184 370.3111
1066 371.3186 370.3113
1067 371.3187 370.3114
1068 371.3187 370.3114
1069 371.3192 370.3119
1070 371.3192 370.3119
1071 372.0509 371.0436
1072 372.0516 371.0443
1073 372.0521 371.0448
1074 372.0523 371.0450
1075 372.0527 371.0454
1076 373.2122 372.2049
1077 373.2821 372.2748
1078 374.2190 373.2117
1079 374.2924 373.2851
1080 374.2928 373.2855
1081 374.2930 373.2857
1082 374.2931 373.2858
1083 374.2932 373.2859
1084 374.2932 373.2859
1085 374.2933 373.2860
1086 374.2943 373.2870
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