MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
887 360.2025 359.1952
888 360.2030 359.1957
889 360.2030 359.1957
890 360.2034 359.1961
891 360.2389 359.2316
892 360.2390 359.2317
893 360.2391 359.2318
894 360.2405 359.2332
895 361.1664 360.1591
896 361.2200 360.2127
897 361.2201 360.2128
898 361.2202 360.2129
899 361.2206 360.2133
900 362.2190 361.2117
901 362.7847 361.7774
902 363.1047 362.0974
903 363.1424 362.1351
904 363.1427 362.1354
905 363.1429 362.1356
906 363.1434 362.1361
907 363.1435 362.1362
908 363.1781 362.1708
909 363.1785 362.1712
910 363.1790 362.1717
911 363.1791 362.1718
912 363.1792 362.1719
913 363.1796 362.1723
914 363.1804 362.1731
915 363.1805 362.1732
916 363.1807 362.1734
917 363.1808 362.1735
918 363.1812 362.1739
919 363.1812 362.1739
920 363.1816 362.1743
921 363.1819 362.1746
922 363.1894 362.1821
923 364.2335 363.2262
924 364.2338 363.2265
925 364.2344 363.2271
926 364.2351 363.2278
927 365.1580 364.1507
928 365.1582 364.1509
929 365.1583 364.1510
930 365.1585 364.1512
931 365.1587 364.1514
932 365.1588 364.1515
933 365.1588 364.1515
934 365.1592 364.1519
935 365.1595 364.1522
936 365.1600 364.1527
937 365.1612 364.1539
938 365.1613 364.1540
939 365.1939 364.1866
940 365.1946 364.1873
941 365.1947 364.1874
942 365.1948 364.1875
943 365.1949 364.1876
944 365.1949 364.1876
945 365.1951 364.1878
946 365.1953 364.1880
947 365.1956 364.1883
948 365.1958 364.1885
949 365.1962 364.1889
950 365.1962 364.1889
951 365.1964 364.1891
952 365.1964 364.1891
953 365.1964 364.1891
954 365.1964 364.1891
955 365.1965 364.1892
956 365.1969 364.1896
957 365.1969 364.1896
958 365.1972 364.1899
959 365.2151 364.2078
960 365.2162 364.2089
961 365.2164 364.2091
962 365.2169 364.2096
963 365.2172 364.2099
964 365.2174 364.2101
965 365.2177 364.2104
966 367.1366 366.1293
967 367.1375 366.1302
968 367.1376 366.1303
969 367.1380 366.1307
970 367.1382 366.1309
971 367.1388 366.1315
972 367.1390 366.1317
973 367.1734 366.1661
974 367.1736 366.1663
975 367.1736 366.1663
976 367.1736 366.1663
977 367.1741 366.1668
978 367.1744 366.1671
979 367.1745 366.1672
980 367.1745 366.1672
981 367.1747 366.1674
982 367.1747 366.1674
983 367.1755 366.1682
984 367.1755 366.1682
985 367.1762 366.1689
986 367.1766 366.1693
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