MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
787 351.1809 350.1736
788 351.1812 350.1739
789 351.1985 350.1912
790 351.1988 350.1915
791 351.1990 350.1917
792 351.1990 350.1917
793 351.1994 350.1921
794 351.2156 350.2083
795 351.2158 350.2085
796 351.2162 350.2089
797 351.2164 350.2091
798 351.2168 350.2095
799 351.2168 350.2095
800 351.2168 350.2095
801 351.2169 350.2096
802 351.2172 350.2099
803 351.2176 350.2103
804 351.2187 350.2114
805 351.2372 350.2299
806 351.2377 350.2304
807 351.2377 350.2304
808 351.2378 350.2305
809 351.2387 350.2314
810 352.9699 351.9626
811 352.9712 351.9639
812 353.1207 352.1134
813 353.1214 352.1141
814 353.1219 352.1146
815 353.1222 352.1149
816 353.1227 352.1154
817 353.1227 352.1154
818 353.1578 352.1505
819 353.1585 352.1512
820 353.1585 352.1512
821 353.1587 352.1514
822 353.1588 352.1515
823 353.1591 352.1518
824 353.1591 352.1518
825 353.1592 352.1519
826 353.1599 352.1526
827 353.1601 352.1528
828 353.1937 352.1864
829 353.2520 352.2447
830 353.2531 352.2458
831 353.2531 352.2458
832 353.2534 352.2461
833 353.2534 352.2461
834 353.2534 352.2461
835 353.8352 352.8279
836 354.0414 353.0341
837 354.0417 353.0344
838 355.0477 354.0404
839 355.0710 354.0637
840 355.0710 354.0637
841 355.0712 354.0639
842 355.0717 354.0644
843 355.0721 354.0648
844 355.1083 354.1010
845 355.1365 354.1292
846 355.1366 354.1293
847 355.1370 354.1297
848 355.1375 354.1302
849 355.1377 354.1304
850 355.1379 354.1306
851 355.1384 354.1311
852 355.1745 354.1672
853 355.1746 354.1673
854 355.1748 354.1675
855 355.1749 354.1676
856 355.1751 354.1678
857 355.8561 354.8488
858 355.8563 354.8490
859 356.0562 355.0489
860 356.0563 355.0490
861 356.0573 355.0500
862 356.1910 355.1837
863 356.2819 355.2746
864 356.2820 355.2747
865 356.2820 355.2747
866 356.2824 355.2751
867 356.2824 355.2751
868 356.2826 355.2753
869 356.2828 355.2755
870 356.2832 355.2759
871 356.2834 355.2761
872 356.9161 355.9088
873 357.0869 356.0796
874 357.0871 356.0798
875 357.9537 356.9464
876 358.0363 357.0290
877 358.0369 357.0296
878 358.0369 357.0296
879 358.1915 357.1842
880 358.2250 357.2177
881 359.1114 358.1041
882 359.1121 358.1048
883 359.1125 358.1052
884 360.0514 359.0441
885 360.0528 359.0455
886 360.2023 359.1950
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