MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
687 344.9205 343.9132
688 345.1774 344.1701
689 345.1842 344.1769
690 345.2252 344.2179
691 345.2256 344.2183
692 345.8939 344.8866
693 346.0361 345.0288
694 346.2231 345.2158
695 346.2232 345.2159
696 346.2234 345.2161
697 346.2234 345.2161
698 346.2235 345.2162
699 346.2238 345.2165
700 346.2240 345.2167
701 346.2245 345.2172
702 346.2248 345.2175
703 346.2257 345.2184
704 347.1837 346.1764
705 347.1837 346.1764
706 347.1840 346.1767
707 347.1844 346.1771
708 347.1846 346.1773
709 347.1849 346.1776
710 347.1854 346.1781
711 347.1854 346.1781
712 347.1854 346.1781
713 347.1856 346.1783
714 347.1856 346.1783
715 347.1859 346.1786
716 347.1862 346.1789
717 347.1863 346.1790
718 347.1863 346.1790
719 347.1863 346.1790
720 347.2039 346.1966
721 347.9287 346.9214
722 348.2019 347.1946
723 348.2024 347.1951
724 348.2027 347.1954
725 348.2029 347.1956
726 348.2031 347.1958
727 348.2033 347.1960
728 349.1272 348.1199
729 349.1625 348.1552
730 349.1628 348.1555
731 349.1633 348.1560
732 349.1636 348.1563
733 349.1639 348.1566
734 349.1641 348.1568
735 349.1643 348.1570
736 349.1647 348.1574
737 349.1657 348.1584
738 349.1659 348.1586
739 349.1990 348.1917
740 349.1991 348.1918
741 349.1992 348.1919
742 349.1992 348.1919
743 349.1994 348.1921
744 349.1995 348.1922
745 349.2000 348.1927
746 349.2004 348.1931
747 349.2004 348.1931
748 349.2005 348.1932
749 349.2008 348.1935
750 349.2008 348.1935
751 349.2009 348.1936
752 349.2012 348.1939
753 349.2012 348.1939
754 349.2013 348.1940
755 349.2013 348.1940
756 349.2013 348.1940
757 349.2014 348.1941
758 349.2018 348.1945
759 349.2021 348.1948
760 349.2214 348.2141
761 349.2216 348.2143
762 349.2218 348.2145
763 349.2219 348.2146
764 349.2220 348.2147
765 349.2221 348.2148
766 349.2236 348.2163
767 349.6878 348.6805
768 351.1423 350.1350
769 351.1425 350.1352
770 351.1427 350.1354
771 351.1427 350.1354
772 351.1427 350.1354
773 351.1433 350.1360
774 351.1436 350.1363
775 351.1437 350.1364
776 351.1438 350.1365
777 351.1787 350.1714
778 351.1788 350.1715
779 351.1788 350.1715
780 351.1788 350.1715
781 351.1789 350.1716
782 351.1790 350.1717
783 351.1793 350.1720
784 351.1794 350.1721
785 351.1799 350.1726
786 351.1804 350.1731
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