MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
587 337.1289 336.1216
588 337.1290 336.1217
589 337.1292 336.1219
590 337.1626 336.1553
591 337.1629 336.1556
592 337.1629 336.1556
593 337.1631 336.1558
594 337.1632 336.1559
595 337.1633 336.1560
596 337.1633 336.1560
597 337.1636 336.1563
598 337.1638 336.1565
599 337.1638 336.1565
600 337.1640 336.1567
601 337.1641 336.1568
602 337.1650 336.1577
603 337.1655 336.1582
604 337.1659 336.1586
605 337.1826 336.1753
606 337.1826 336.1753
607 337.1828 336.1755
608 337.1830 336.1757
609 337.1832 336.1759
610 337.1835 336.1762
611 337.1841 336.1768
612 337.2011 336.1938
613 337.2572 336.2499
614 338.1826 337.1753
615 338.6707 337.6634
616 338.9879 337.9806
617 339.0168 338.0095
618 339.1049 338.0976
619 339.1051 338.0978
620 339.1057 338.0984
621 339.1063 338.0990
622 339.1414 338.1341
623 339.1417 338.1344
624 339.1426 338.1353
625 339.1427 338.1354
626 339.1427 338.1354
627 339.1427 338.1354
628 339.1428 338.1355
629 339.1429 338.1356
630 339.1433 338.1360
631 339.1436 338.1363
632 339.1438 338.1365
633 339.1439 338.1366
634 339.1787 338.1714
635 339.1792 338.1719
636 339.1794 338.1721
637 339.1794 338.1721
638 339.1796 338.1723
639 339.1799 338.1726
640 339.1820 338.1747
641 339.1822 338.1749
642 339.1989 338.1916
643 339.2000 338.1927
644 340.9832 339.9759
645 341.0552 340.0479
646 341.0917 340.0844
647 341.1196 340.1123
648 341.1208 340.1135
649 341.1212 340.1139
650 341.1216 340.1143
651 341.1580 340.1507
652 341.1581 340.1508
653 341.1581 340.1508
654 341.1583 340.1510
655 341.1583 340.1510
656 341.1584 340.1511
657 341.1588 340.1515
658 341.1593 340.1520
659 341.1595 340.1522
660 341.1596 340.1523
661 341.1958 340.1885
662 341.1973 340.1900
663 341.2159 340.2086
664 342.0406 341.0333
665 342.0406 341.0333
666 342.0410 341.0337
667 342.0414 341.0341
668 342.0415 341.0342
669 342.1910 341.1837
670 342.1918 341.1845
671 342.1921 341.1848
672 342.1926 341.1853
673 343.2091 342.2018
674 343.2101 342.2028
675 343.2101 342.2028
676 343.2964 342.2891
677 343.8958 342.8885
678 344.0209 343.0136
679 344.0564 343.0491
680 344.0573 343.0500
681 344.2075 343.2002
682 344.2075 343.2002
683 344.2080 343.2007
684 344.2083 343.2010
685 344.2098 343.2025
686 344.2102 343.2029
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