MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
487 329.1595 328.1522
488 329.1596 328.1523
489 329.9559 328.9486
490 330.0408 329.0335
491 330.0414 329.0341
492 330.0414 329.0341
493 330.0415 329.0342
494 330.0773 329.0700
495 330.1925 329.1852
496 330.1928 329.1855
497 330.8642 329.8569
498 330.8645 329.8572
499 331.1761 330.1688
500 331.1766 330.1693
501 331.1899 330.1826
502 331.2105 330.2032
503 331.2346 330.2273
504 331.8860 330.8787
505 332.0306 331.0233
506 332.0563 331.0490
507 332.2071 331.1998
508 332.2076 331.2003
509 332.2076 331.2003
510 332.2084 331.2011
511 332.2084 331.2011
512 333.0561 332.0488
513 333.0565 332.0492
514 333.1326 332.1253
515 333.1693 332.1620
516 333.1891 332.1818
517 333.1891 332.1818
518 333.1894 332.1821
519 333.1898 332.1825
520 333.2047 332.1974
521 333.2050 332.1977
522 333.2057 332.1984
523 333.2057 332.1984
524 333.2059 332.1986
525 333.2060 332.1987
526 333.2061 332.1988
527 333.2062 332.1989
528 333.2062 332.1989
529 333.2063 332.1990
530 333.2068 332.1995
531 333.2248 332.2175
532 333.2250 332.2177
533 333.9638 332.9565
534 334.0102 333.0029
535 334.1870 333.1797
536 334.1873 333.1800
537 334.2248 333.2175
538 334.2256 333.2183
539 334.9587 333.9514
540 334.9593 333.9520
541 334.9595 333.9522
542 335.1111 334.1038
543 335.1472 334.1399
544 335.1474 334.1401
545 335.1475 334.1402
546 335.1476 334.1403
547 335.1476 334.1403
548 335.1478 334.1405
549 335.1480 334.1407
550 335.1482 334.1409
551 335.1483 334.1410
552 335.1487 334.1414
553 335.1488 334.1415
554 335.1490 334.1417
555 335.1497 334.1424
556 335.1849 334.1776
557 335.1854 334.1781
558 335.1856 334.1783
559 335.1857 334.1784
560 335.1857 334.1784
561 335.1857 334.1784
562 335.1868 334.1795
563 335.2205 334.2132
564 335.2207 334.2134
565 335.2214 334.2141
566 335.2214 334.2141
567 335.2217 334.2144
568 335.2217 334.2144
569 335.2219 334.2146
570 335.2219 334.2146
571 335.2220 334.2147
572 335.2221 334.2148
573 335.2225 334.2152
574 335.2410 334.2337
575 335.9727 334.9654
576 335.9788 334.9715
577 335.9795 334.9722
578 337.0341 336.0268
579 337.1255 336.1182
580 337.1267 336.1194
581 337.1269 336.1196
582 337.1271 336.1198
583 337.1271 336.1198
584 337.1272 336.1199
585 337.1281 336.1208
586 337.1284 336.1211
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